Phalaenopsis orchid miniaturization by overexpression of OsGA2ox6, a rice GA2-oxidase gene

Botanical Studies

Table 1 Morphological comparison of leaves and roots in nontransgenic (NT) and GA2ox6-OX transgenic lines

	NT	1-1^a	3-2	3-3	3-4
Leaf length^b (cm)	17.6 ± 3.2	5.3 ± 1.0^**	6.8 ± 0.5^**	5.8 ± 0.5^**	7.9 ± 0.3^**
Leaf width^c (cm)	6.2 ± 0.9	6.0 ± 0.6	5.2 ± 0.5	5.5 ± 0.4	5.8 ± 0.3
Leaf L/W ratio^d	2.9 ± 0.9	0.9 ± 0.1^**	1.3 ± 0.2^**	1.1 ± 0.1^**	1.4 ± 0.1^**
Root length^e (cm)	54.9 ± 16.6	14.3 ± 4.3**	13.3 ± 8.8^**	19.3 ± 11.6^**	21.8 ± 1.6^**
Root width^f (cm)	1.8 ± 0.1	2.1 ± 0.2**	2.8 ± 0.4^**	3.1 ± 0.4^**	2.4 ± 0.3^**

Three plants each from the NT line and four GA2ox6-OX transgenic lines (#1-1, #3-2, #3-3 and #3-4) were measured and compared
^a Plants of line #1-1 were grown in 9 cm pots at a relative early stage (Additional file 1: Fig S5). Others were grown in 10.5 cm pots
^b Leaf length average ± SE of the longest leaf from 3 individual plants
^c Leaf width average ± SE of the same longest leaf from the above 3 individual plants
^d Ratio of leaf length to leaf width
^e Root length average ± SE of the longest root from 3 individual plants
^f Root width average ± SE of the thickest root from 3 individual plants
** Average ± SE (n = 3) showed a significant difference from the NT control with a p-value < 0.01