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Botanical Studies
Fig. 5 | Botanical Studies

Fig. 5

From: Sequence analysis and protein interactions of Arabidopsis CIA2 and CIL proteins

Fig. 5

Interactions between CIA2/CIL and CO, as assessed by Y2H assay. Vectors pAS2-1 and pACT2 harbor GAL4 BD and GAL4 AD, respectively. The CIA2 and CIL fragments were ligated into pAS2-1 vector. The full-length CO fragment was ligated into pACT2 vector. Colonies were assayed for SD/-Leu-Trp autotrophy, SD/-Leu-Trp-Trp + 20 or 50 mM 3-AT autotrophy, and β-galactosidase activity. pACT2, negative control. PC, positive control containing pVA3-1 and pTD1-1

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