Table 2 Gene-specific primer sequences of CIA2-interacting proteins used in this study
From: Sequence analysis and protein interactions of Arabidopsis CIA2 and CIL proteins
Protein (Restriction enzyme) | Forward primers (5′ to 3′)a | Reverse primers (5′ to 3′) |
|---|---|---|
ABI3 (BamHI and SalI) | CGGGATCCCGATGAAAAGCTTGCATGTGGC | GCGTCGACTCATTTAACAGTTTGAGAAGTTGG |
ARR3 (NcoI and BamHI) | CGCCATGGCCAAAGACGGTGGCGTTTC | CGGATCCCTAAGCTAATCCGGGACTCCTC |
CO (SmaI and BamHI) | CGCCCGGGGATGTTGAAACAAGAGAGTAACGAC | GCGGATCCTCAGAATGAAGGAACAATCCCATATC |
CIA2 (NcoI and SmaI) | CGCCATGGAGATGTCGGCGTGTTTAAGCAGCG | CGCCCGGGTTATCTTTGTCCACTTGGAGTG |
CIL (NcoI and SmaI) | CGCCATGGAGATGTCCTCTTGTGCCTATAGC | CGCCCGGGTAATCGTAGACCACTTAAATTCC |
NF-YB1 (NcoI and EcoRI) | CATGCCATGGCGGATACGCCTTCGAG | GGAATTCTTACCAGCTCGGCATTTCTTCA |
NF-YC1 (NcoI and EcoRI) | CATGCCATGGATACCAACAACCAGCAAC | GGAATTCTTAACCTTGGCCGTCGAG |
NF-YC9 (BamHI and SalI) | CGGGATCCGAATGGATCAACAAGACCATGG | GCGTCGACTAATTTTCCTGGTCAGGTTGG |