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Fig. 1 | Botanical Studies

Fig. 1

From: Silencing of PhLA, a CIN-TCP gene, causes defected petal conical epidermal cell formation and results in reflexed corolla lobes in petunia

Fig. 1

Schematic representation of PhLA cDNA and phylogram of its sequence with other reported TCPs. a cDNA structure of PhLA. Relative positions of PhLA open reading frame and the 5′ and 3′ UTR are indicated. The blue box marks the region encoding TCP domain and the yellow line shows the location of miR319 recognition site. Two fragments used for VIGS analysis are indicated in green, and the black line shows the probe region for in situ hybridization. Arrows show location and direction of primers used to measure transcript abundance. b Phylogenetic tree for TCP genes. Class II TCPs were divided into CYC/TB subclade and CIN subclade. PhLA was grouped in CIN subclade and is most closely related to SlLA in all genes analyzed. The transcripts containing a miR319 recognition site are underlined. For sequences used in this study, their GenBank accession numbers are listed in “Materials and methods

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