Skip to main content
Fig. 2 | Botanical Studies

Fig. 2

From: Reductions of copper ion-mediated low-density lipoprotein (LDL) oxidations of trypsin inhibitors, the sweet potato root major proteins, and LDL binding capacities

Fig. 2

a Effects of SPTIs on oxLDL binding were evaluated by protein stains in native PAGE gels with two layers of separation gels. The selected square frame was quantified by a Syngene G:bBOX imaging system (Syngene, UK), and b the HPLC equipped with TSKgel G3000PWXL (7.8 × 300 mm) gel filtration column was used to analyze the molecular size changes of SPTIs/oxLDL complex. (1) The SPTIs alone in glycine–HCl buffer (pH 2.0, deep blue line); (2) SPTIs/oxLDL binding assays in the glycine–HCl buffer (pH 2.0, green line); (3) SPTIs/oxLDL binding assays in the distilled water (red line). Values were presented as mean ± SD and were analyzed using one-way ANOVA, followed by a post hoc Tukey’s test for multiple comparisons. The different marked symbols in each bar were significantly different (P < 0.05)

Back to article page