Fig. 2From: Reductions of copper ion-mediated low-density lipoprotein (LDL) oxidations of trypsin inhibitors, the sweet potato root major proteins, and LDL binding capacitiesa Effects of SPTIs on oxLDL binding were evaluated by protein stains in native PAGE gels with two layers of separation gels. The selected square frame was quantified by a Syngene G:bBOX imaging system (Syngene, UK), and b the HPLC equipped with TSKgel G3000PWXL (7.8 × 300 mm) gel filtration column was used to analyze the molecular size changes of SPTIs/oxLDL complex. (1) The SPTIs alone in glycine–HCl buffer (pH 2.0, deep blue line); (2) SPTIs/oxLDL binding assays in the glycine–HCl buffer (pH 2.0, green line); (3) SPTIs/oxLDL binding assays in the distilled water (red line). Values were presented as mean ± SD and were analyzed using one-way ANOVA, followed by a post hoc Tukey’s test for multiple comparisons. The different marked symbols in each bar were significantly different (P < 0.05)Back to article page