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Botanical Studies

Table 1 Validation of the transcription levels of selected unigenes by qRT-PCR

From: Physiological and transcriptome changes induced by exogenous putrescine in anthurium under chilling stress

Unigene ID Description Fold change a qPT-PCR b
Response to cold unigenes
 TRINITY_DN10807 Protein ESKIMO 1-like [XP_020276425.1] 5.45 6.27 ± 0.12
 TRINITY_DN13429 Annexin D8 [XP_010939366.1] 10.21 12.74 ± 0.74
Plasma membrane unigenes
 TRINITY_DN10426 CASP-like protein 1E1 [XP_026663055.1] 19.16 16.41 ± 0.68
 TRINITY_DN23828 Cytokinin hydroxylase-like [XP_008787566.2] 24.56 18.68 ± 1.07
Peroxidase activity unigenes
 TRINITY_DN15525 Peroxidase [PON83694.1] 10.95 14.98 ± 1.15
 TRINITY_DN22642 Cationic peroxidase 1 [XP_006842568.2] 11.13 9.08 ± 0.27
Proline metabolism unigenes
 TRINITY_DN13650 Arginine decarboxylase-like [XP_009418323.1] 3.23 2.85 ± 0.10
 TRINITY_DN16024 Prolyl 4-hydroxylase 9 [XP_009397284.1] 4.62 2.78 ± 0.25
  1. a Foldchange, ratio(S1/S2). S1, the FPKM value of a unigene under Put treatment; S2, the FPKM value of a unigene in control group
  2. b qRT-PCR, relative transcription level of unigenes under Put treatment measured by qRT-PCR compared to that of control group
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