Physiological and transcriptome changes induced by exogenous putrescine in anthurium under chilling stress

Botanical Studies

Table 1 Validation of the transcription levels of selected unigenes by qRT-PCR

Unigene ID	Description	Fold change ^a	qPT-PCR ^b
Response to cold unigenes
TRINITY_DN10807	Protein ESKIMO 1-like [XP_020276425.1]	5.45	6.27 ± 0.12
TRINITY_DN13429	Annexin D8 [XP_010939366.1]	10.21	12.74 ± 0.74
Plasma membrane unigenes
TRINITY_DN10426	CASP-like protein 1E1 [XP_026663055.1]	19.16	16.41 ± 0.68
TRINITY_DN23828	Cytokinin hydroxylase-like [XP_008787566.2]	24.56	18.68 ± 1.07
Peroxidase activity unigenes
TRINITY_DN15525	Peroxidase [PON83694.1]	10.95	14.98 ± 1.15
TRINITY_DN22642	Cationic peroxidase 1 [XP_006842568.2]	11.13	9.08 ± 0.27
Proline metabolism unigenes
TRINITY_DN13650	Arginine decarboxylase-like [XP_009418323.1]	3.23	2.85 ± 0.10
TRINITY_DN16024	Prolyl 4-hydroxylase 9 [XP_009397284.1]	4.62	2.78 ± 0.25

^a Foldchange, ratio(S1/S2). S1, the FPKM value of a unigene under Put treatment; S2, the FPKM value of a unigene in control group
^b qRT-PCR, relative transcription level of unigenes under Put treatment measured by qRT-PCR compared to that of control group