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Fig. 9 | Botanical Studies

Fig. 9

From: Asymbiotic germination of Vanilla planifolia in relation to the timing of seed collection and seed pretreatments

Fig. 9

Protocorm development and seedling growth of V. planifolia. a By 30 days of culture, a few embryos had emerged from the seed coat. Scale bar = 1 mm. b Young protocorms with numerous rhizoids appeared at the basal protocorms by 60 days of culture. Scale bar = 1 mm. c The protocorm had enlarged with the differentiation of a shoot tip by 90 days of culture. Scale bar = 1 mm. d The protocorm had a differentiated first root by 120 days of culture. Scale bar = 1 mm. e By 150 days of culture, the protocorm had elongated with the prominent root formation, ready to transfer to the growth medium. Bar = 2 mm. f After 90 days of culture on the growth medium, the seedlings grew into vines with several healthy roots. Bar = 2 cm

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