Cys-SH based quantitative redox proteomics of salt induced response in sugar beet monosomic addition line M14

Li, Jinna; Wang, Kun; Ji, Meichao; Zhang, Tingyue; Yang, Chao; Liu, He; Chen, Sixue; Li, Hongli; Li, Haiying

doi:10.1186/s40529-021-00320-x

Botanical Studies

Table 1 List of 28 up-regulated redox proteins from sugar beet M14 leaves between control and NaCl treatment (all the entries with p < 0.05 from three biological replicates)

From: Cys-SH based quantitative redox proteomics of salt induced response in sugar beet monosomic addition line M14

No	Protein ID^a	Description	Plant species	Sequence with modification^b	iodoTMTsalt200 /control Ratio^c	iodoTMTsalt400 /control Ratio^d	p-value	iTRAQ salt200 /control Ratio^f	iTRAQ salt400 /control Ratio^g	Function^e
1	G1E6K5	Carbonic anhydrase	Dimocarpus longan	FMVFAC²¹⁰SDSR	–	1.40	0.01	–	1.09	Metabolism (3)
2	A0A0K9RGC9	Beta-galactosidase	Spinacia oleracea	YWPTFGPQC⁶⁵³NLYVPAPLLR	–	1.20	0.03	–	1.01
3	A0A2P6UZB2	Triosephosphate chloroplastic	Micractinium conductrix	VIAC¹⁰⁷³VGETLEQR	–	1.40	0.01	–	0.93
4	Q8MC96	ATP synthase epsilon chain	Apium graveolens	TLNLC⁵VLTPNR	2.40	–	0.00	–	0.81	Transport (3)
5	A0A1S2XUR4	GDSL esterase/lipase At5g33370	Cicer arietinum	VLVTGTGPLGC²³⁰VPGELASQ GSQNGEC²⁴⁵APEPQR		1.66	0.01	–	0.31
6	A0A1U7VX65	GDSL esterase/lipase At5g45670-like	Nicotiana sylvestris	FALIGIGQIGC²²¹SPNQLAQRSP DGATC²³⁹DDTVNSANR	–	1.52	0.01	–	0.31
7	C0Z387	AT2G21660 protein	Arabidopsis thaliana	C¹⁰FVGGLAWATDDR	–	1.48	0.01	–	1.07	Biosynthesis (5)
8	A0A0K9QZ36	Biotin carboxyl carrier protein of acetyl-CoA carboxylase	Spinacia oleracea	QYDC¹⁴⁷ELLIR	–	1.21	0.01	–	1.11
9	A0A1J3HHY8	Glutamate-1-semialdehyde 2,1-aminomutase	Noccaea caerulescens	FVNSGTEAC²⁰⁰MGVIR	–	1.57	0.04	–	1.18
10	O24365	Chloroplast mRNA-binding protein CSP41	Spinacia oleracea	LC³²⁵AQATGR	–	1.94	0.01	–	0.95
11	O50036	Heat shock 70 protein	Spinacia oleracea	FEELC³⁷⁷SDLLDR	–	1.31	0.00	–	1.12
12	A0A1D1Y6P4	Vacuolar-sorting receptor 2	Anthurium amnicola	YC²⁸⁶APDPEQDFSR	–	1.22	0.02	–	1.08	Signal transduction (2)
13	A0A1R3GZ43	EGF-like calcium-binding protein	Corchorus olitorius	YC²⁶⁹APDPEQDFSR	–	1.22	0.01	–	1.00	Signal transduction (2)
14	A0A161DY72	DUF642	Vitis quinquangularis	SDDFSSLC³⁵²GPVIDDVR; VAEIMIHNPGVEEDPAC¹⁷³GPLIDSVAMR	–	1.37	0.04	–	0.80	Stress and defense (1)
15	A0A0K9QD73	Profilin	Spinacia oleracea	TGQALVIGLYDEPVTPGQC¹¹⁷NMIVER	–	1.32	0.02	–	0.77	Transcription (1)
16	P10871	Ribulose bisphosphate carboxylase/oxygenase activase	Spinacia oleracea	MC²²³ALFINDLDAGAGR	1.51	1.48	0.03	1.09	1.07	Photosynthesis (7)
17	A0A0K9QU20	Fructose-bisphosphate aldolase	Spinacia oleracea	TVVSVPC¹⁹⁷GPSALAVKEAAWGLAR	–	1.20	0.00	0.94	0.92
18	A0A1U8EH95	Ribulose bisphosphate carboxylase/oxygenase activase 2	Capsicum annuum	KGNMC⁴⁷⁰VLFINDLDAGAGR	–	1.78	0.03	–	1.05
19	O20252	Sedoheptulose-1,7-bisphosphatase	–	LFC²⁵⁵PGNLR	–	1.47	0.04	–	0.87
20	P09559	Phosphoribulokinase	Spinacia oleracea	FFNPVYLDEGSTISWIPC²⁹⁶GR	–	1.33	0.04	–	1.08
21	P12355	Photosystem I reaction center subunit III	Spinacia oleracea	FENYGNYGLLC¹³⁹GSDGLPHLIVSGDQR	–	1.20	0.01	–	0.80
22	P10871	Ribulose bisphosphate carboxylase/oxygenase activase	Spinacia oleracea	IGVC³¹⁶TGIFR	–	1.26	0.00	–	1.00
23	A0A0K9QDU1	Peroxidase	Spinacia oleracea	NSFYASTC³¹PGVEGIVR	–	1.49	0.03	–	0.67	ROS homeostasis (2)
24	A0A1J6IUE1	Thioredoxin-like 3–1	Nicotiana attenuata	ENSQPIIIDWMANWC¹⁰⁸R	–	1.43	0.01	–	1.06	ROS homeostasis (2)
25	A0A0K9R8D4	Uncharacterized protein LOC104907026	–	AGQFC¹¹⁷GGFTAIER	0.38	1.47	0.00	1.01	0.95	Unknown (4)
26	A0A068TKJ7	Uncharacterized protein	–	YTEGFSGADITEIC⁴⁷⁸QR	–	1.45	0.02	–	0.76
27	A0A0J8CV41	Chalcone-flavonone isomerase family protein	Beta vulgaris subsp. vulgaris	TLPEEILNSIIGETGVC¹⁹⁹PQAR	–	1.45	0.02	–	1.14
28	A0A0K9R8D4	Uncharacterized protein	–	AGQFC¹¹⁷GGFTAIER	–	1.47	0.00	–	0.98

^aProtein ID, gi number of NCBI
^b Sequence with modification, the lower case letter are phosphorylation site in each peptide
^csalt200/control Ratio, a relative abundance of proteins at redox peptide level ( 200 mM NaCl treatment versus control), P-value < 0.05
^dsalt400/control Ratio, a relative abundance of proteins at redox peptide level ( 400 mM NaCl treatment versus control), P-value < 0.05
^eFunction, according to Blast2GO software
^fsalt200/control Ratio, a relative abundance of proteins at total protein level ( 200 mM NaCl treatment versus control), P-value < 0.05
^gsalt400/control Ratio, a relative abundance of proteins at total protein level ( 400 mM NaCl treatment versus control), P-value < 0.05. The number in brackets, indicate the numbers of proteins in corresponding function

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