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Botanical Studies

Table 2 The results of response of PSV with three viability tests of epiphytic and terrestrial orchids

From: Seed viability testing for research and conservation of epiphytic and terrestrial orchids

Response

Predictor

Estimate

Std. Error

CI

Z value

P- value

(a) Epiphytic and Terrestrial orchids

     

PSV

(Intercept)

2.48

0.29

1.91 – 3.05

8.52

 < 0.001

 

FDA test vs. EB test

−0.78

0.15

−1.08 – -0.48

−5.1

 < 0.001

 

TTC test vs. EB test

−3.02

0.17

−3.34 – -2.70

−18.29

 < 0.001

 

FDA test vs. TTC test

2.24

0.16

1.93 – 2.55

14.2

 < 0.001

 

Sterilized vs. non-sterilized seeds

-0.85

0.15

−1.15 – -0.55

−5.57

 < 0.001

 

Terrestrial vs. epiphyte lifeform

−0.82

0.26

−1.33 – -0.31

−3.17

0.002

 

Stored vs. fresh seeds

0.08

0.23

−0.36 – 0.53

0.37

0.712

 

FDA test*sterilized and not sterilized seeds vs. EB test *sterilized and not sterilized seeds

−0.17

0.21

−0.58 – 0.24

−0.8

0.422

 

TTC test*sterilized and not sterilized seeds vs. EB test*sterilized and not sterilized seeds

1.62

0.22

1.20 – 2.04

7.52

 < 0.001

 

FDA test*sterilized and not sterilized seeds vs. TTC test*sterilized and not sterilized seeds

−1.79

0.21

−2.20 – -1.38

−8.49

 < 0.001

 

AIC = 5941.644; Marginal R2 = 0.251; Conditional R2 = 0.284

     

(b) Epiphytic orchids

     

PSV

(Intercept)

2.27

0.33

1.62 – 2.92

6.82

 < 0.001

 

FDA test vs. EB test

−1.1

0.32

−1.72 – -0.48

−3.47

0.001

 

TTC test vs. EB test

−2.13

0.32

-2.77 – -1.49

−6.57

 < 0.001

 

FDA test vs. TTC test

1.03

0.29

0.46 – 1.60

3.57

 < 0.001

 

Sterilized vs. Not sterilized seeds

−1.01

0.31

−1.63 – -0.40

−3.22

0.001

 

Stored vs. Fresh seeds

0.07

0.27

−0.45 – 0.59

0.26

0.795

 

FDA test*sterilized and not sterilized seeds vs. EB test*sterilized and not sterilized seeds

0.47

0.42

−0.37 – 1.30

1.1

0.272

 

TTC test*sterilized and not sterilized seeds vs. EB test*sterilized and not sterilized seeds

1.44

0.42

0.62 – 2.27

3.42

0.001

 

FDA test*sterilized and not sterilized seeds vs. TTC test*sterilized and not sterilized seeds

−0.98

0.4

−1.76 – -0.19

−2.44

0.015

 

AIC = 1589.915; Marginal R2 = 0.123; Conditional R2 = 0.132

     

(c) Terrestrial orchids

     

PSV

(Intercept)

1.74

0.19

1.37 – 2.10

9.26

 < 0.001

 

FDA test vs. EB test

-0.68

0.17

−1.02 – -0.35

−3.99

 < 0.001

 

TTC test vs. EB test

-3.42

0.2

−3.81 – -3.03

−17.15

 < 0.001

 

FDA test vs. TTC test

2.74

0.19

2.36 – 3.12

14.2

 < 0.001

 

Sterilized vs. Not sterilized seeds

-0.8

0.17

−1.14 – -0.46

−4.62

 < 0.001

 

Stored vs. Fresh seeds

0.1

0.29

−0.47 – 0.67

0.34

0.73

 

FDA test*sterilized and not sterilized seeds vs. EB test*sterilized and not sterilized seeds

-0.38

0.24

−0.84 – 0.08

−1.61

0.108

 

TTC test*sterilized and not sterilized seeds vs. EB test*sterilized and not sterilized seeds

1.75

0.26

1.25 – 2.25

6.84

 < 0.001

 

FDA test*sterilized and not sterilized seeds vs. TTC test*sterilized and not sterilized seeds

-2.13

0.25

−2.62 – -1.64

−8.49

 < 0.001

 

AIC = 4332.92; Marginal R2 = 0.279; Conditional R2 = 0.319

     
  1. The results of the response of probability of finding viable seeds (PSV) with three viability tests; Evans blue test (EB), Fluorescein diacetate test (FDA) and Tetrazolium test (TTC), with and without sterilization, using Beta-Binomial models, of (a) epiphytic and terrestrial orchids, (b) epiphytic orchids and (c) terrestrial orchids. The bold font values are significant at P < 0.05
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