Fig. 10

Deduced protein sequence alignment of nag1 and nag2, and expression of nag1 and TvmNAG2. A The deduced TvmNAG2, TvNag1, TvNag2 and TrNag sequences from T. virens strain mango, T. virens strain Gv29-8, T. virens strain Gv29-8 and T. reesei strain QM6a were aligned (accession number of OL456168, XM_014095216, XM_014099474, and XM_006963001, respectively). The conserved E (Glu) and D (Asp) in active site were highlight. Two amino acids of deduced TvmNAG2 marked in red were different from the amino acids within the matched TvNag2 peptides of MALDI/MS analyses. Such peptides were boxed. Putative glycosylated sites, NxS/T were shaded. B The expression of TvmNAG2 and nag1 from T. virens strain mango in liquid and solid cultures were analyzed by RT-PCR. Line 1 and 4, actin; line 2 and 5, nag1; line 3 and 6. TvmNAG2