Fig. 3

Optimal pH and temperature of TvmNAG2. A To determine the optimal pH of TvmNAG2, NAGase activity assay was performed at various pH of buffer (final 41.7 mM) including citrate buffer, acetate buffer, Tris buffer or phosphate buffer at 65 °C for 30 min. B To determine the optimal temperature of TvmNAG2, NAGase activity assay was performed at different temperature, 30–80 °C for 30 min at pH 5