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Fig. 5 | Botanical Studies

Fig. 5

From: Purification, identification and characterization of Nag2 N-acetylglucosaminidase from Trichoderma virens strain mango

Fig. 5

TLC and HPLC analyses of product hydrolyzed by TvmNAG2 using colloidal chitin as the substrate. A TvmNAG2 was incubated with 1.5% colloidal chitin at 30–60 °C for 20 h, followed by TLC analysis (left). TvmNAG2 was treated at 4, 40, 50, 60 and 70 °C for 2 h. Afterward, TvmNAG2 was incubated with 1.5% colloidal chitin at 40 °C for 20 h, followed by TLC analysis (middle). diGlcNAc (N, N’-diacetylchitibiose), GlcNAc and mixture (diGlcNAc and GlcNAc) was on TLC analysis as well (right). B TvmNAG2 was incubated with 1.5% colloid chitin at 37 °C for 20 h, followed by HPLC analyses (upper). For comparison, GlcNAc was used as the standard sample. The colloid chitin was as the control (below)

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