Table 1 Purification of NAGase from T. virens. T. virens strain mango were cultured in a liquid medium containing chitin for 8 days, followed by the purification steps, including ammonium sulfate precipitation, and a chitin-bead affinity chromatography, followed by NAGase activity assay using pNP-NAG as substrate
Procedure | Total volume (ml) | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Purification (folds) | Yield (%) |
|---|---|---|---|---|---|---|
Culture filtrate | 450.0 | 2,430.60 | 8.820 | 275.6 | 1.0 | 100.00 |
(NH4)2SO4 precipitation | 1.9 | 3,772.80 | 2.420 | 1,558.6 | 5.7 | 155.00 |
Chitin-bead chromatography | 60.0 | 64.19 | 0.006 | 10,698.3 | 38.8 | 2.64 |