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Table 2 Properties of the reported NAGase from various Trichoderma spp.

From: Purification, identification and characterization of Nag2 N-acetylglucosaminidase from Trichoderma virens strain mango

Trichoderma spp.

Name of enzyme; form; expression level

Mw (kDa)

Optimal pH; optimal temperature

pH tolerance;

temperature tolerance

(% activity remained)

Specify activityb

(U mg−1 min−1);

purification folds

Kmb

Refs.

T. virens

strain mango

TvmNAG2; native; 5.4 U/ml

 ~ 68

pH 5

(pH 5–6, > 90% activity); 60–65 °C

pH 4–9 at 25 °C for 60 min (80%);

60 °C at pH 7 for 120 min (~ 85%)

10,698.3; 38.8

0.45 mM

In this study

T. reesei

RutC30ΔU3

Nag1; Recombinant;

499.85 U/ml

 ~ 80

pH 4

(pH 3.5–6, > 60% activity); 60 °C

pH 4–6 for 60 min (80%);

60 °C for 120 min (~ 80%), 60 °C for 8 h (~ 60%)

319.89

69.41 ± 4 M

(Chen et al. 2015)

T. harzianum

1051/ NAGase

NAGase

36

pH 4; 37 °C

nd;

50 °C at pH 4 for 60 min ~ 50%

nd

8.06 mM

(De Marco et al. 2004)

T. harzianum

Strain 39.1

NAGase; native

64,

118a

pH 5.6; 50 °C

nd

nd

1.27 µmol

(Ulhoa et al. 2001)

T. harzianum

T198

Exochitinase;

native

27.5 ~ 28

pH 3.5; 50 °C

pH 2–8 at 37 °C for 60 min (50%);

60 °C at pH 5.5 for 60 min (33%)

nd

850 µM c

(Deane et al. 1998)

T. harzianum P1

Nag1;

native

72

nd

nd

11.8bd; 9

nd

(Lorito et al. 1994)

T. harzianum AF6-T8

NAGase; native; 3.1 U/ml

 ~ 69,

150a

pH 4–5.5; 50 °C

pH 3–6 at 37 °C for 60 min (80%);

65 °C at pH 5.2 for 30 min (~ 50%)

102; 13.2

0.24 mM

(Koga et al. 1991)

  1. nd no data
  2. aEstablished by gel filtration
  3. bpNP-NAG as substrate
  4. cChitotriose as substrate
  5. dSpecific activity = nkatal mg−1; one nkatal corresponds to the release of 1 nmol of p-nitrophenol per second