Table 1 The PCR primers, optimal annealing temperatures, and product sizes of ITS, trnL-trnF, and trnH-psbA regions in this study
DNA regions | Primers | Primer Sequences (5′–> 3′) | Primer sources | Annealing temperature (°C) | Product size (bp) |
|---|---|---|---|---|---|
Nuclear region | |||||
ITS (ITS1 + 5.8S + ITS2) | ITS_4 ITS_5 | TCCTCCGCTTATTGATATGC GGAAGTAAAAGTCGTAACAAGG | White et al. 1990 White et al. 1990 | 55 | 662–666 |
ITS_L ITS_Scleria | TCGTAACAAGGTTTCCGTAGGTG ATGCTTAAACTCAGCGGGTA | Hsiao et al. 1994 Liu et al. 2021 | 60 | 662–666 | |
Chloroplast regions | |||||
trnL-trnF | trnLF_c trnLF_f | CGAAATCGGTAGACGCTACG ATTTGAACTGGTGACACGAG | Taberlet et al. 1991 Taberlet et al. 1991 | 60 | 829–895 |
trnLF_c trnLF_d | CGAAATCGGTAGACGCTACG GGGGATAGAGGGACTTGAAC | Taberlet et al. 1991 Taberlet et al. 1991 | 60 | 466–471 | |
trnLF_f trnLF_e | ATTTGAACTGGTGACACGAG GGTTCAAGTCCCTCTATCCC | Taberlet et al. 1991 Taberlet et al. 1991 | 59 | 369–383 | |
trnH-psbA | psbAF trnH2 | GTTATGCATGAACGTAATGCTC CGCGCATGGTGGATTCACAATCC | Sang et al. 1997 Tate and Simpson 2003 | 64 | 460–494 |
trnH2 psbABL | CGCGCATGGTGGATTCACAATCC AGCTGCTTGGCCTGTAGTAG | Tate and Simpson 2003 This study | 63 | 505–637 | |