Table 2 Primers used for RT-qPCR
From: Seed Halopriming Improves Salinity Tolerance of Some Rice Cultivars During Seedling Stage
Gene | Primer sequences (5′3′) | Number of bases | % GC content | Tm (°C) | Product size (kb) |
|---|---|---|---|---|---|
OsSOS1 | F: acgcaaggcaatagaagagg | 20 | 50.00 | 59.48 | 164 |
R: ttggctggtccaacaattac | 20 | 45.00 | 58.48 | ||
OsNHX1 | F: cgggatgattggtttgttct | 20 | 45.00 | 59.79 | 128 |
R: cccgccaactaaagatggta | 20 | 50.00 | 59.95 | ||
OsHKT1 | F: gctcaaggccttcacaaaag | 20 | 50.00 | 59.99 | 152 |
R: ggcccaattagaaacctgaa | 20 | 45.00 | 59.02 | ||
UBIQUITIN | F: cacaagaaggtgaagctcgc | 20 | 55.00 | 62.00 | 183 |
| Â | R: ctctctggttgtagacgtagg | 21 | 52.00 | 64.00 |