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Table 1 Disinfection and scarification treatment of vanilla seeds prior to sowing

From: Acid scarification as a potent treatment for an in vitro germination of mature endozoochorous Vanilla planifolia seeds

Subject of disinfection

State of seeds

70% ethanol

Disinfectant

Time

Concentration

Tween-20

Pepsin

Sterile deionized water

Seeds

Mature

4 min

H2SO4

5 min

96%

No

No

3x

Seeds

Mature

4 min

H2SO4

15 min

96%

No

No

3x

Seeds

Mature

4 min

H2SO4

30 min

96%

No

No

3x

Seeds

Mature

4 min

HCl

30 min

0.1 M

Yes

Yes

3x

Seeds

mature

4 min

HCl

30 min

0.1 M

No

No

3x

Seeds

Mature

4 min

HCl

4 h

0.1 M

Yes

Yes

3x

Seeds

Mature

4 min

HCl

15 min

35 – 38%

No

No

3x

Seeds

Mature

4 min

H2SO4

3 min

50%

No

No

3x

Seeds

Mature

4 min

H2SO4

washed

50%

No

No

3x

Seeds

Mature

4 min

H2SO4

5 min

50%

No

No

3x

Seeds

Mature

4 min

Chlorinated lime

30 min

66 g/l

No

No

3x

Fruits

Immature

no

Chlorinated lime

5 min

20% w/v

Yes

No

1x

  1. Disinfection and scarification treatment of Vanilla planifolia seeds prior to sowing. Tween-20 (1 drop per 200 ml) and pepsin (0.5 g/100 ml, porcine gastric mucosa, 250 U/mg, Sigma-Aldrich) were added into disinfection solution of selected treatments as indicated