Characterization of soil organic matter in perhumid natural cypress forest: comparison of humification in different particle-size fractions
© Chen et al.; licensee Springer. 2013
Received: 18 January 2012
Accepted: 5 July 2013
Published: 15 November 2013
The Chamaecyparis forest is a valuable natural resource in eastern Asia. The characteristics of soil humic substances and the influence of environmental factors in natural Chamaecyparis forests in subtropical mountain regions are poorly understood. The study site of a perhumid Chamaecyparis forest is in the Yuanyang Lake Preserved Area in northcentral Taiwan. We collected samples from organic horizons (Oi, Oe and Oa) and from the surface horizon (O/A horizon) at the summit, footslope and lakeshore to characterize the composition of the soil organic matter. Samples of organic horizons were dried and ground, and those of the O/A horizon were passed through wet sieving for different particle-size fractions before analysis. The C chemical structure in the samples was determined with CP/MAS 13C NMR spectra.
The ratios of alkyl-C/O-alkyl-C and aromaticity increased with decomposition of litter from the Oi, Oe, to Oa horizon. The ratio of alkyl-C/O-alkyl-C also increased from coarse (> 250 μm) to very fine (< 2 μm) particle fractions, which indicates increased humification of soil organic matter (SOM) in the fine-sized fractions. However, aromaticity tended to decrease with decreasing particle size, so it may not be useful in evaluating SOM humification of different particle-size fractions.
The humification degree of the samples from O horizons and different particle-size fractions of the O/A horizon showed no gradient change with change in topography. This prevalent slow decomposition of organic matter in these perhumid climate conditions may narrow the difference in humification from the summit to lakeshore.
KeywordsCP/MAS 13C NMR Perhumid forest Particle-size fraction Soil organic matter Humification degree
Humus substances are the most recalcitrant and major fraction of soil organic matter (SOM). The humus in soils has beneficial effects on plant nutrient supply, soil structure and water-holding capacity. Because of high stability, humus benefits carbon storage in soils. However, decomposition of humus releases CO2, which may contribute to increased atmospheric CO2 level and the greenhouse effect (Piccolo, 1996).
Chamaecyparis cypress forest is a valuable natural resource in eastern Asia for its high-quality timber. As well, the accumulation of non-decomposed organic matter on the forest floor provides a critical buffer to retain water and prevent soil erosion with heavy rainfall in montane areas. The dead timber and thick SOM might store a previously underestimated large C pool. Several decades ago, before large-scale logging, Chamaecyparis forest was widely distributed in cloudy montane areas in Taiwan, at about 800 to 2800 m a.s.l.
Much effort has been invested in investigating soil properties and fertility management of Japanese hinoki cypress (Chamaecyparis obtusa) plantations (e.g., Inagaki et al. 2008;2011). By contrast, characteristics of humic substances under natural Chamaecyparis forests in this subtropical montane area are not well known because most of the preserved natural Chamaecyparis forests are located in steep and remote areas with poor access to roads. The Chi-Lan Mountain contains one of the few natural preserved Chamaecyparis forests in northcentral Taiwan.
Podzolic soils are commonly found under Chamaecyparis forests in the cold and humid subalpine region in Taiwan (Chiu et al. 1999; Jien et al. 2010a, b2013). The extremely high acidity and soil moisture in such undisturbed Chamaecyparis forest results in low diversity of soil bacterial communities (Lin et al. 20102011) as well as low decomposition of organic matter and humification degree of humic acids (Chung et al. 2012). Our previous studies revealed that the topography and intrinsic properties of different fractions of organic matter in a mountain lake environment affect the distribution and migration of organic substances (Chen and Chiu 2000). The degree of humification of SOM decreased slightly from the summit to lakeshore, and the relatively low degree of humification was due to high precipitation and acidity (Chung et al. 2012).
Particle-size fractionation has been widely used to study the physical and chemical properties and decomposition of SOM (Joliveta et al. 2006; Muñoz et al. 2009). Our previous study indicated that the humification degree of SOM increased from coarse to fine fraction size in humid subalpine soils (Chen and Chiu 2003). We hypothesized that SOM humification might be hampered differently by varied decomposition with different particle-size fractions, with possible interactions of SOM characteristics, particle-size fractions and soil moisture conditions. We aimed to clarify the differences in organic matter composition in various particle-size fractions as affected by topography in a Chamaecyparis forest.
Study site and sampling
The Yuanyang Lake ecosystem (24°35’N, 121°24’E) is located in the northcentral part of Taiwan from 1670 to 2169 m a.s.l. This region is a cloud forest system because of annual precipitation of 4000 mm and annual mean temperature 13°C, with a temperate perhumid climate. This area was selected as a representative long-term ecological study site of Taiwan. The vegetation is dominated by Hinoki cypress (C. obtusa) and Taiwan false cypress (C. formosensis) with an understory evergreen broadleaf shrub (Rhododendron formosanum).
The forest soils were characterized as Albaquult, Dystrochrept and Histosol in the summit, footslope and lakeshore regions, respectively (Chiu et al. 1999; Chen and Chiu 2000). The organic matter at the summit and footslope is mor type, which is a thick mat of non-decomposed to partially decomposed O horizons, and humic peat type at the lakeshore with continuous water saturation, for a moderately decomposed class of peat characterized by 1/3 to 2/3 recognizable plant fibers. Soil at the summit is well drained, whereas that at the footslope and lakeshore is poorly drained. In particular, the lakeshore, located about 1.5 m above the lake, frequently experiences inundation of water during the monsoon season.
We selected 3 representative pedons along a topographic sequence at the summit, footslope and lakeshore. At each study site, a pit was excavated for describing macromorphological soil characteristics and for collecting soil samples according to standard procedures (Soil Survey Staff 2006). The toposequence for clay mineralogical characterization in this study site has been described elsewhere (Pai et al. 2007). Organic horizons (i.e., Oi, Oe and Oa) were classified and collected on the basis of rubbed fiber content that can be identified by eyes and fingers.
In addition, we collected the O/A horizon for comparing particle-size fractions. Three replicate subsamples were collected from each topographic position with use of a soil auger 8 cm in diameter and 10 cm deep before being bulked together. Non-decomposed and partially decomposed plant litter was removed before sampling.
Physical and chemical analyses
Soil samples first underwent low-energy sonication and then were separated into different particle-size fractions by a combination of wet sieving and continuous flow centrifugation. Four particle-size fractions, including coarse (> 250 μm), medium (53–250 μm), fine (2–53 μm), and very fine (< 2 μm), were separated (Chen and Chiu 2003). All fractionated samples were freeze-dried and stored. Samples collected from Oi, Oe, and Oa horizons were dried at 70°C and ground for analyses.
Solid-state CP/MAS 13C NMR determination involved use of a Bruker DSX 400 MHz instrument operating at 100.46 MHz and spin rate 7 kHz. The 0.5-g soil sample was used each time. Acquisition parameters were contact time, 1 ms; pulse delay time, 1 s; and spectra plotted region, 0 to 200 ppm (Chen and Chiu 2003; González-Pérez et al. 2008; Novak and Smeck 1991). About 10,000 scans were collected for samples (Quideau et al. 2001). The C functional groups were determined by the following chemical-shift areas (Novak and Smeck 1991; Chen and Chiu 2003; Jien et al. 2011): alkyl-C signals, 0 to 50 ppm; O-alkyl-C signals, 50 to 90 ppm; di-O-alkyl C signals, 90 to 110 ppm; aromatic-C signals, 110 to 165; and carboxyl-C signals, 165 to 190 ppm. The methoxyl-C of the chemical shift signal appeared at 56 ppm and overlapped with the O-alkyl-C signals (Knicker 2000). The signal intensities in the respective chemical shift regions are expressed as a percentage of the total spectra area. The relative contents of different chemical structures were calculated from the area under the spectra. To compare each spectrum for samples, we chose the peak of carboxyl C as the standard peak and standardized the signals by adjusting the peak area to the same size for each sample. Then, we compared the peaks of different functional groups in terms of relative percentage of each functional C group. The area for each functional C group peak was calculated by use of Topspin software for the NMR instrument.
The above ratios were used to evaluate the humification degree and decomposition of SOM.
The total C (TC) and total N (TN) contents in the samples from O horizons and samples of particle-size fractions from the O/A horizon were determined after combustion in a Nitrogen Analyzer (NA1500 Series 2, Fisons, Italy).
Chemical characteristics and 13C NMR analyses
The chemical properties of partially decomposed materials collected at various organic horizons in 3 topographic positions of the Yuanyang Lake ecosystem in Taiwan
Total C, N content and C/N ratio in each particle-size fraction of soils collected from the O/A horizon at 3 topographic positions
Medium (250 ~ 53)
Fine (53 ~ 2)
Very Fine (< 2)
Medium (250 ~ 53)
Fine (53 ~ 2)
Very Fine (< 2)
Medium (250 ~ 53)
Fine (53 ~ 2)
Very Fine (< 2)
Relative distribution of integrated peak areas of chemical shift regions in the CP/MAS 13 C NMR spectra of partially decomposed materials collected from 3 organic horizons at 3 topographic positions
C functional group (%)
A/ O-A ratioa
13C NMR analyses of particle-size fractions of O/A horizon
Particle-size distribution of soils collected from the O/A horizon (0–10 cm) at 3 topographic positions
Particle size (μm)
Very fine (< 2)
Relative intensities of the CP/MAS 13 C NMR spectra in different particle-size fractions isolated from the O/A horizon (0–10 cm) in 3 topographic positions
C functional groups (%)
A/ O-A ratioa
Medium (250 ~ 53)
Fine (53 ~ 2)
Very fine (<2)
Medium (250 ~ 53)
Fine (53 ~ 2)
Very fine (<2)
Medium (250 ~ 53)
Fine (53 ~ 2)
Very fine (<2)
Samples from 3 organic horizons of the Yuanyang Lake ecosystem predominantly consisted of organic matter. The high content of C in these horizons was consistent with that in other perhumid forests (Mafra et al. 2007; Schawe et al. 2007). Poor drainage and flooding in riparian soils diminishes the decomposition of SOM but improves the denitrification by microorganisms (Mafra et al. 2007). Thus, we found lower TN content and higher C/N ratio at the lakeshore than footslope and summits (Table 1). The C/N ratio varied markedly by particle-size fraction at the summit but not greatly at the footslope and lakeshore (Table 2). High total organic C content throughout the fractions indicates the accumulation of organic matter in the soil. In addition, high percentages (61% to 83%) of samples were in the coarse fraction with decomposing plant residues (Table 4), which suggests the retardation of organic matter decomposition in such perhumid climate conditions.
Carbon functional groups in different particle-size fractions
We found O-alkyl-C and di-O-alkyl-C as the dominant components in the entire soil fraction, which can probably be used by microorganisms during humification processes (Keeler et al. 2006). The region of aromatic-C includes phenolic-C, derived from lignin and tannin, and bacterial resynthesized compounds consisting of alkyl-C and carboxyl-C (Mahieu et al. 1999; Mathers et al. 2000; Ussiri and Johnson 2003; López et al. 2008).
Physical fractionation of soil according to particle size followed by chemical, biological, and physical analyses of fractions is a powerful tool in process-oriented SOM research (Mao et al. 2007). Particle-size fractioning allows for separating SOM pools of varying degrees of microbial alteration and mineral association (Joliveta et al. 2006). The SOM in the coarse fraction primarily consisted of labile plant residues, whereas microbial biomass is supposed to be concentrated in the very fine fraction (Zech et al. 1996; Kimetu et al. 2008). Thus, SOM associated with very fine fractions tends to be more aliphatic than does whole SOM (Mao et al. 2007; Jiménez et al. 2008; Muñoz et al. 2009). We found low C/N ratio (Table 2) and high alkyl-C content (Table 5) in very fine particle-size fractions, which suggests the loss of easily decomposable carbohydrates and selective preservation of inherently recalcitrant materials during the plant residue decomposition (Mathers et al. 2000; Wagai et al. 2008; Rovira et al. 2009).
The O-alkyl-C content in coarse and medium fractions was higher at the lakeshore than at the summit and footslope. In particular, peaks at 56, 63, 84 and 89 ppm (O-alkyl-C) were completely diminished from coarse (>250 μm) to very fine (<2 μm) at the summit, with only a trace amount at the footslope and lakeshore (Figure 2). The O-alkyl-C content might be greater at the lakeshore than other positions and was universally lower in fine than coarse particles (Table 5), as was found in our previous study of subalpine forest and grassland soils (Chen and Chiu 2003). Thus, the spectra of the coarse fraction (>250 μm) resembled that of decomposing materials (Figures 1 and 2), which resulted from most of the soils being in a coarse fraction (Table 4).
Humification degree of organic horizons and particle-size fractions of the O/A horizon
The humification degree and characteristics of SOM could be evaluated by ratio of alkyl-C/O-alkyl-C and aromaticity. The high ratio of alkyl-C/O-alkyl-C and aromaticity indicates the high humification degree of SOM in the soils (Baldock et al. 1997; Almendros et al. 2000; Chen et al. 2004; Mueller and Koegel-Knabner 2009). The alkyl-C/O-alkyl-C ratios of the Oi, Oe, and Oa horizons in the 3 positions increased with increasing soil depth (Table 3), which indicates a higher humification degree in the Oa horizon. We found no typical pattern for alkyl-C/O-alkyl-C ratios in O horizons along topographical positions (Table 3), which indicates restrained decomposition and humification because of low temperature and high annual precipitation. The results for aromaticity were similar to those for alkyl-C/O-alkyl-C (Table 3), which could reflect the humification state of SOM. The influence of litter input on changes in remnant masses of each carbon during the early humification processes showed that mass loss rate of aromatic C in humified litter is higher in cypress than cedar (Ono et al. 20092011). Our previous studies showed that humification degree of HAs, determined by E4/E6 ratio (Chen et al. 2001a, b) and by 13C NMR (Chung et al. 2012) differed little by topographic position of the study site.
The ratio of alkyl-C/O-alkyl-C increased with decreasing particle-size fraction (Table 5), so high stability in the fine fraction is due to the protection by clay minerals (Wagai et al. 2008) and to the chemical resistance of the alkyl-C structure to decomposition. The humification degree in the medium and fine fractions of the O/A horizon samples was similar to that in the O horizons in the 3 studied positions, which reflects the chemical properties in SOM being strongly dominated by the distribution of particle-size fractions. The ratios of alkyl-C/O-alkyl-C in each particle-size fraction with topographic change under the Chamaecyparis forest were similar in value and variation and were lower than in Tsuga forest soils (Chen and Chiu 2003). Thus, the relatively low proportion of alkyl-C/O-alkyl-C ratios is due to the poor decomposition under perhumid climate conditions.
By comparison, aromaticity findings were opposite to those for ratios of alkyl-C/O-alkyl-C in particle-size fraction. Aromaticity decreased with decreasing particle size (Table 5). The present results agree with the suggestions of Hempfling et al. (1987) and Almendros et al. (2000 (2000) questioning the increase in aromaticity during humification in soils. The results of Baldock et al. (1997) also showed that aromatic C contents tended to decrease with decreasing particle size, which suggests that increased extent of decomposition was not accompanied by an increase in aromatic C content. If aromatic C content did not accumulate with decomposition, an increase in aromaticity should not be used as an indicator of the extent of decomposition.
The distribution of particle-size fractions in soil is a critical factor in determining the chemical composition of SOM. 13C NMR analysis of soil particle-size fractions revealed that the C structure of the SOM changes with different particle-size fraction. The accumulation of recalcitrant C compounds in the fine particle-size fraction was contributed by alkyl-C rather than aromatic-C. The ratio of alkyl-C/O-alkyl-C indicates increasing SOM humification with decreasing particle size. The humification degree showed no gradient change pattern in different topographical positions. The effect of topography on the decomposition and humification of organic matter in the Chamaecyparis forest was apparently overshadowed by the slow decomposition of organic matter.
This work was supported in part by the National Science Council, Taiwan (NSC 101-2621-B-001-002-MY3).
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