A new Haniffia species (Zingiberaceae) and a new generic record from Sarawak, Malaysian Borneo
© Wong et al.; licensee Springer 2014
Received: 10 March 2014
Accepted: 19 May 2014
Published: 5 June 2014
Haniffia Holttum is a genus of three described species of terrestrial gingers hitherto restricted to Peninsular Thailand and various localities in Peninsular Malaysia.
With generic placement confirmed using nrITS, trn K and mat K plastid sequence data, Haniffia santubongensis S.Y. Wong & P.C. Boyce is described as a taxonomically novel species representing a new generic record for Borneo, to where it is endemic to Mount Santubong, Kuching Division, NW Sarawak, Malaysian Borneo. An identification key to all species is given and H. santubongensis is illustrated from living plants.
Haniffia santubongensis is the fourth species of Haniffia so far described, and the first occurring on sandstone.
KeywordsHaniffia santubongensis Mount Santubong Phylogeny Taxonomy
Haniffia Holttum is a genus of three described species of terrestrial gingers hitherto restricted to Peninsular Thailand and various localities in Peninsular Malaysia. The three described species are all seemingly locally endemic. The type species, H. cyanescens (Ridl.) Holttum, is restricted to Bukit Tanga (Negeri Sembilan, Peninsular Malaysia), with a variety, H. cyanescens var. penangiana C.K. Lim, occurring on Pulau Pinang and Kedah. The most recently recognized species, H. flavescens Y.Y. Sam & Julius (Sam et al. ) is known only from Endau Rompin National Park (Johor, Peninsular Malaysia). The sole extra-Malaysian species, H. albiflora K. Larsen & Mood, is confirmed only from Nam Tok Chatwarin, Naratiwat, Thailand. A summary of the taxonomic history of Haniffia Holttum is presented by Larsen and Mood ().
Fresh leaf material of Haniffia santubongensis was collected from the type locality, Mount Santubong. The type specimen with the spirit material was deposited to SAR.
DNA extraction, amplification and sequencing
List of species included in this study with vouchers (herbarium location) and GenBank accession numbers for DNA sequences used in the phylogenetic analyses
Haniffia albiflora K. Larsen & Mood
Kress #99-6370 (US)
Haniffia cyanescens ( Ridl.) Holttum
Julius et al. FRI56069
Haniffia flavescens Y.Y. Sam & Julius
Julius et al. FRI57598
Haniffia santubongensis S.Y. Wong & P.C. Boyce
P.C. Boyce & S.Y. Wong ZI22
Newmania orthostachys N.S. Lý & Škorničk.
Lý 470 (VNM, E, P, SING)
Newmania serpens N.S. Lý & Škorničk.
Lý 332 (VNM, E, P, SING)
Phylogenetic analyses were performed with PAUP*4.0b10 (Swofford ) for maximum parsimony (MP) reconstruction with all characters equally weighted. The most parsimonious trees were obtained with heuristic searches of 1,000 replicates with random stepwise sequences addition, tree bisection-reconnection (TBR) branch swapping, collapse of zero-length branches, with the multiple-tree option in effect, and saving up to 10,000 trees from each random sequence addition.
The most suitable nucleotide substitution model for each of the gene regions was selected in jModeltest ver. 0.1.1 (Posada ) using Akaike information criterion (AIC). General time reversible (GTR + I + G) was the nucleotide substitution model selected. Maximum likelihood (ML) analyses were carried out using RAxML 7.2.6 (Stamatakis et al. ). Maximum likelihood bootstrap values were obtained by running 10,000 replicates. Bayesian phylogenetic analyses were performed with MrBayes ver. 3.1.2 (Huelsenbeck and Ronquist ). Markov chain Monte Carlo (MCMC) was repeated twice to assure parameter convergence. The MCMC algorithm was run for 2,000,000 generations with one cold and three heated chains, starting from random trees and sampling one out of every 100 generations. Convergence was assessed by using the standard deviation of split frequencies as convergence index with values < 0.005 interpreted as indicating good convergence. The first 10% of trees were discarded as burn-in. Remaining trees were used to construct 50% majority-rule consensus trees.
Results and discussion
Morphology and biogeography
In overall appearance H. santubongensis is most similar to H. cyanescens, sharing with that species a wide bluish labellum and semi-glossy fruits. However, H. santubongensis is clearly distinct from H. cyanescens by the lateral staminodes with an oblique bifid tip, the labellum distally notched (not deeply divided), and differences in labellum colouration and patterning (most notably the presence of a median yellow callus in H. santubongensis).
Haniffia santubongensis represents a new generic record for Borneo where it is locally endemic to Gunung Santubong. Combined with the three locally endemic species in Peninsular Malaysia and P. Thailand, it provides further compelling evidence that these Haniffia species, along with numerous other examples in families as diverse as the aroids, the palms, Rubiaceae Juss., and the genus Hanguana Blume, represent relictual fragments of the Riau Pocket phytochore (Ashton ; Corner ).
Key to Haniffia species
1a. Corolla lobes and staminodes pale yellow; labellum pale yellow with golden yellow median band, apex emarginate. . . . . . . . H. flavescens
1b. Corolla lobes and staminodes white; labellum white, white with purple veins or dark blue-violet; apex bilobed. . . . . . . . . 2
2a. Leaf blade 10–14?×?2–3.5 cm; ligule with long white hairs; flowers 2–5 in each inflorescence. S Thailand. . . . . . . . . H. albiflora
2b. Leaf blade 17–21 × 3–4.9 cm; ligule glabrous or sparsely pubescent; flowers 5–7 in each inflorescence. Malaysia. . . . . . . . . 3
3a. Lateral staminodes bifid at apex; labellum distally notched, with a central yellow median band. NW Borneo. . . . . . . . H. santubongensis
3b. Lateral staminodes not bifid; labellum deeply spit, without a central yellow median band. Peninsular Malaysia. . . . . . . . 4
4a. Labellum dark blue-violet with white veins; lateral staminodes obovate to oblanceolate. . . . . . . . H. cyanescens var. cyanescens
4b. Labellum white with violet veins; lateral staminodes lanceolate. . . . . . . . . . H. cyanescens var. penangiana
Haniffia santubongensis most closely resembles H. cyanescens var. cyanescens (Negeri Sembilan) but is readily distinguished by the oblique-tipped, bifid lateral staminodes, and labellum distally notched (not deeply split) with a yellow median band extending from the entrance to the basal spur to the innermost extent of the lip distal division.
Terrestrial, clumping herb ca 50 cm tall; rhizomes ca 10 mm diam., shallowly buried in soil; roots fibrous. Leafy pseudostem 5–25 per clump, closely spaced, composed of leafless sheaths and leaf sheaths, base ca 1 cm diam.; leafless sheaths ca 6 per shoot, occupying the lower 1/3-1/2, reddish, glabrous, membranous on apex and margins. Leaves 10– 20 per shoot, sub-sessile, sheaths very short, to ca 1 cm long; ligule obtuse to almost truncate, slightly emarginate, ca 3 mm long, soon turning black, glabrous; petiole reddish; lamina narrowly elliptic to lanceolate, 8.5-15 × 2.5-3 cm, base cuneate, apex acuminate, acumen 6–20 mm long, glabrous, bright green, abaxially very slightly paler with deep green slender primary lateral veins. Inflorescences borne at base of leafy shoot, usually solitary, rarely two together, 5–7 cm long, with ca 5 flowers per inflorescence, peduncle to 3.5 cm long, reddish where exposed, otherwise pale green, basal sheaths 1–2, 1–2 cm, pink. Bracts boat-shaped, 15–25 × 6–10 mm, spirally arranged, membranous, reddish green, glabrous, each subtending one flower. Calyx tubular, 20–30 mm long, apex unequally tridentate, greenish, translucent, hairy at tip. Floral tube 36–70 mm long, greenish white, slightly glandular-hairy inside and out; dorsal corolla lobe elliptic, 20–26 × ca. 6 mm, membranous, greenish white with darker veins, lateral corolla lobes narrowly elliptic, 20–25 × ca 4 mm, membranous, greenish white with darker veins, glabrous. Lateral staminodes spatulate, apex oblique bifid, 15–20 × 4–6 mm, greenish white with darker veins, both surfaces covered with dense, greenish white glandular hairs. Labellum obovate, 21–25 × 10–14 mm, swollen at base where adnate to staminodes, apex distally notched, distal lobes curved downwards, white with blue staining, lobes suffused purple or dark blue-violet, centrally with a yellow median callus, upper surface covered with dense, white glandular hairs, lower surface sub-glabrous. Stamen white, covered with dense, glandular hairs; filament 5–6 mm long; anther 6–10 mm long; anther crest 2–2.5 mm long, apex bidentate, white. Style 46–54 mm long, glabrous; stigma cup-shaped, ca1.8 mm long, ciliate. Ovary 3-locular, ovules many. Epigynous glands two, free, linear, 2–2.4 mm long, yellow. Fruit a capsule, dehiscent, somewhat 3-sided, globose with prominent terminal floral remains, ca. 2 × 2 cm, semi-glossy brownish, heavily stained with reddish brown speckles on a cream base, exterior very sparsely warty, splitting longitudinally into 3 valves, valves fleshy, usually with 3-locules well developed. Seeds ellipsoid-obovoid, 6–7 × 3–4 mm, glossy brown, turning greyish-black, aril thick, white when fresh.
Partially shaded, deep sandy peat podzols of ridge kerangas in Dryobalanops-dominated hill forest; ca 200–250 m asl.
Haniffia santubongensis is known only from the type locality where it occurs as two separate, dense, populations.
The species epithet is derived from the name of the type locality.
Haniffia santubongensis is the fourth species of Haniffia and represents a new generic record for Borneo.
This study was funded by Ministry of Education Malaysia through Niche Research Grant Scheme No. NRGS/1089/2013-(03). Fieldwork and research has most recently been under Research Permit No. NCCD.907.4.4(Jld.9)-69 and Park Permit No. 140/2013. We also sincerely thanked the two referees for their valuable comments.
- Ashton PS: Lambir’s forest: The world’s most diverse known tree assemblage? In Pollination Ecology and The Rain Forest: Sarawak Studies. Edited by: Roubik DW, Sakai S, Hamid Karim AA. Springer, New York; 2005:191–216. 10.1007/0-387-27161-9_17View ArticleGoogle Scholar
- Corner EJH: The Malayan flora. In Proceedings of the Centenary and Bicentenary Congress of Biology. Edited by: Purchon RD. University of Singapore, Singapore; 1960:21–24.Google Scholar
- Drummond AJ, Ashton B, Buxton S, Cheung M, Cooper A, Duran C, Heled J, Kearse M, Markowitz S, Moir R, Stones-Havas S, Sturrock S, Swidan F, Thierer T, Wilson A: Geneious v5.6. 2012.Google Scholar
- Edgar RC: MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 2004, 32: 1792–1797. 10.1093/nar/gkh340View ArticlePubMedPubMed CentralGoogle Scholar
- Huelsenbeck JP, Ronquist F: MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 2001, 17: 754–755. 10.1093/bioinformatics/17.8.754View ArticlePubMedGoogle Scholar
- Larsen K, Mood J: Revision of the genus Haniffia (Zingiberaceae). Nord J Bot 2000, 20: 285–289. 10.1111/j.1756-1051.2000.tb00745.xView ArticleGoogle Scholar
- Leong-Škorničková J, Lý N-S, Poulsen AD, Tosh J, Forrest A: Newmania : a new ginger genus from central Vietnam. Taxon 2011, 60: 1386–1396.Google Scholar
- Posada D: jModelTest: phylogenetic model averaging. Mol Biol Evol 2008, 25: 1253–1256. 10.1093/molbev/msn083View ArticlePubMedGoogle Scholar
- Sam YY, Julius A, Chew MY: Haniffia flavescens (Zingiberaceae): a new species from Peninsular Malaysia. Bot Stud 2009, 50: 359–364.Google Scholar
- Stamatakis A, Hoover P, Rougemont J: A rapid bootstrap algorithm for the RAxML web-servers. Syst Biol 2008, 75: 758–771. 10.1080/10635150802429642View ArticleGoogle Scholar
- Swofford DL: PAUP*: Phylogenetic Analysis Using Parsimony (* and other methods) Version 4.0 Beta 10. Sinauer Associates, Sunderland; 2002.Google Scholar
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