The transformation vector and the molecular and phenotype analysis of the Ubi:OsMADS45 transgenic rice. (A) A schematic diagram of the transformation vector, Ubi:OsMADS45. Full-length OsMADS45 cDNA driven by an ubiquitin (Ubi) promoter, and the nos terminator was inserted into the plasmid vector, pCAMBIA1301. (B) Southern blot analysis of four selected Ubi:OsMADS45 transgenic plants. Total genomic DNA isolated from four transgenic lines (45OX1, 45OX2, 45OX4, and 45OX5) was digested with Nco I and hybridized with a GUS DNA probe. (C) RT-PCR analysis of Ubi:OsMADS45 transgenic rice. Total RNA was isolated from 20 DAI leaves and analyzed using PCR primers specific for the OsMADS45 gene. Actin expression was assessed as an internal control. (D) The plant heights of TNG67 rice and the four transgenic lines from 20 to 100 DAI. The heights of 12-16 plants for each line were measured, and the error bars indicate the SE of the mean at each time point. (E) Morphological characteristics of TNG67 rice, non-transgenic (NT) and transgenic lines at 74 DAI. Arrowheads indicate the flowering panicles. Inset: an enlargement from the square portion indicated in line 1. DAI, days after imbibition; GUS, beta-glucuronidase; and HPT, hygromycin phosphor transferase.