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Botanical Studies
Figure 3 | Botanical Studies

Figure 3

From: Improving initial infectivity of the Turnip mosaic virus (TuMV) infectious clone by an mini binary vector via agro-infiltration

Figure 3

Infectivity assay of pBD-TuMV-YC5, pBD-TuMV-GFP and pBD-TuMV-GK. (A) The 3 constructs were directly mechanically introduced to C. quinoa plants, and local lesions appeared on the inoculated leaves at 7 dpi (upper panel). The virus culture of TuMV YC5 and Mock-inoculation were used as positive and negative controls, respectively. Bar, 0.5 cm. The GFP fluorescence expressed by pBD-TuMV-GFP and pBD-TuMV-GK is shown in the lower panel. Bar, 25 μM. (B) N. benthamiana plants were infiltrated with agrobacterium individually carrying pBD-TuMV-YC5, pBD-TuMV-GFP, and pBD-TuMV-GK. The virus culture of TuMV YC5 and Mock-inoculation were used as positive and negative controls, respectively. The photographs were taken at 14 dpi (upper panel). GFP expression was detected with a fluorescent microscope (lower panel).

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