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Table 2 The germination rates of seeds of B. formosana using different periods of cryostorage

From: Cryopreservation the seeds of a Taiwanese terrestrial orchid, Bletilla formosana (Hayata) Schltr. by vitrification

Seeds Cryopreservation period1
0 min 10 min 20 min 30 min 1 week 1 Year
TTC staining (%)2 77 94 94 93 96
Germination rate (%)3 79b 89a 87a 89a 87a 90a
  1. 1Seeds were immersed in loading solution for 30 min and PVS2 for 30 min.
  2. 2Approximately 300–350 seeds were tested in each TTC-staining.
  3. 3Values are means of 5 replicates (200–300 seeds per replicate). Means followed by the same letters are not significantly different by Duncan’s test (P=0.05). Germination rates were calculated after 4 weeks of culture.