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Botanical Studies

Table 2 The germination rates of seeds of B. formosana using different periods of cryostorage

From: Cryopreservation the seeds of a Taiwanese terrestrial orchid, Bletilla formosana (Hayata) Schltr. by vitrification

Seeds

Cryopreservation period1

0 min

10 min

20 min

30 min

1 week

1 Year

TTC staining (%)2

77

94

94

93

96

–

Germination rate (%)3

79b

89a

87a

89a

87a

90a

  1. 1Seeds were immersed in loading solution for 30 min and PVS2 for 30 min.
  2. 2Approximately 300–350 seeds were tested in each TTC-staining.
  3. 3Values are means of 5 replicates (200–300 seeds per replicate). Means followed by the same letters are not significantly different by Duncan’s test (P=0.05). Germination rates were calculated after 4 weeks of culture.
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