Seeds
|
Cryopreservation period1
|
---|
0 min
|
10 min
|
20 min
|
30 min
|
1 week
|
1 Year
|
---|
TTC staining (%)2
|
77
|
94
|
94
|
93
|
96
|
–
|
Germination rate (%)3
|
79b
|
89a
|
87a
|
89a
|
87a
|
90a
|
- 1Seeds were immersed in loading solution for 30 min and PVS2 for 30 min.
- 2Approximately 300–350 seeds were tested in each TTC-staining.
- 3Values are means of 5 replicates (200–300 seeds per replicate). Means followed by the same letters are not significantly different by Duncan’s test (P=0.05). Germination rates were calculated after 4 weeks of culture.