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Table 3 List of RAPD primers, their sequence, optimal annealing temperature (T m ) and banding pattern of both mother plant and field-grown micropropagated plants of Aloe vera

From: High frequency microcloning of Aloe vera and their true-to-type conformity by molecular cytogenetic assessment of two years old field growing regenerated plants

Sl. No.

Prime

Sequence 5′-3′

Tm(°C)

Total Bands

1

OPA-09

GGGTAACGCC

41

5

2

OPA-16

AGCCAGCGAA

38

10

3

OPC-06

GAACGGACTC

41

6

4

OPG-08

TCACGTCCAC

38

4

5

OPG-10

AGGGCCGTCT

41

4

6

OPJ-04

CCGAACACGG

41

4

7

OPK-10

GTGCAACGTG

41

6

8

OPL-02

TGGGCGTCAA

41

9

9

OPL-04

GACTGCACAC

41

4

10

OPL-05

ACGCAGGCAC

41

3

11

OPM-06

CTGGGCAACT

41

10

12

OPN-15

CAGCGACTGT

38

6

13

OPN-18

GGTGAGGTCA

38

4

14

OPAC-07

GTGGCCGATG

38

3

15

OPAC-20

ACGGAAGTGG

38

4

Total

   

82