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Table 1 Effect of plant growth regulators on callus induction and shoot regeneration of leaf explants of Hygrophila pogonocalyx Hayata

From: Chemical and pharmacological investigation of micropropagated Hygrophila pogonocalyx produced from leaf explants

Plant growth regulators (mg/l) 4-week-culture
Callus induction (%)
8-week culture
Shoot no./explant Mean stem length (cm)
BA (0.1) + NAA (0.1) 100 6.2 ± 0.8d* 1.8 ± 0.2a
BA (0.5) + NAA (0.1) 100 12.2 ± 1.9b 0.8 ± 0.1d
BA (1.0) + NAA (0.1) 100 3.8 ± 1.3e 0.7 ± 0.1d
BA (0.1) + IAA (0.1) 100 6.6 ± 1.1d 1.4 ± 0.2b
BA (0.5) + IAA (0.1) 100 22.8 ± 1.9a 1.0 ± 0.1c
BA (1.0) + IAA (0.1) 100 9.2 ± 1.3c 0.8 ± 0.1d
BA (0.1) + IAA (0.1) + 2iP (1) 100 7.2 ± 0.8d 1.7 ± 0.2a
BA (0.5) + IAA (0.1) + 2iP (1) 100 11.4 ± 1.1b 1.0 ± 0.2c
BA (1.0) + IAA (0.1) + 2iP (1) 100 9.0 ± 0.7c 0.7 ± 0.1d
  1. *: In a single column, means with the same letter are not significantly different according to Duncan’s multiple range tests at the 0.05 level.