Chemical and pharmacological investigation of micropropagated Hygrophila pogonocalyx produced from leaf explants

Botanical Studies

Table 1 Effect of plant growth regulators on callus induction and shoot regeneration of leaf explants of Hygrophila pogonocalyx Hayata

Plant growth regulators (mg/l)	4-week-culture Callus induction (%)	8-week culture
Plant growth regulators (mg/l)	4-week-culture Callus induction (%)	Shoot no./explant	Mean stem length (cm)
BA (0.1) + NAA (0.1)	100	6.2 ± 0.8^d*	1.8 ± 0.2^a
BA (0.5) + NAA (0.1)	100	12.2 ± 1.9^b	0.8 ± 0.1^d
BA (1.0) + NAA (0.1)	100	3.8 ± 1.3^e	0.7 ± 0.1^d
BA (0.1) + IAA (0.1)	100	6.6 ± 1.1^d	1.4 ± 0.2^b
BA (0.5) + IAA (0.1)	100	22.8 ± 1.9^a	1.0 ± 0.1^c
BA (1.0) + IAA (0.1)	100	9.2 ± 1.3^c	0.8 ± 0.1^d
BA (0.1) + IAA (0.1) + 2iP (1)	100	7.2 ± 0.8^d	1.7 ± 0.2^a
BA (0.5) + IAA (0.1) + 2iP (1)	100	11.4 ± 1.1^b	1.0 ± 0.2^c
BA (1.0) + IAA (0.1) + 2iP (1)	100	9.0 ± 0.7^c	0.7 ± 0.1^d

*: In a single column, means with the same letter are not significantly different according to Duncan’s multiple range tests at the 0.05 level.