Effect of pH (6.0 and 7.0) on dehydroascorbate reductase activity. Purified recombinant protein of MT-I and MT-II were with (A) or without (B) 4 mM glutathione in the reaction mixtures. The reaction was carried out at 30°C by adding 100 μL MT-I and MT-II solution (100 μg protein, 100 mM phosphate buffer, pH 7.0 and 6.0) to 0.9 mL DHA solution with or without 4 mM glutathione. The increase of absorbance at 265 nm was recorded for 5 min. The experiments were done twice and a representative one is shown.