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Botanical Studies
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Figure 4 | Botanical Studies

Figure 4

From: Dehydroascorbate reductase and monodehydroascorbate reductase activities of two metallothionein-like proteins from sweet potato (Ipomoea batatas [L.] Lam. ‘Tainong 57’) storage roots

Figure 4

Effect of pH (6.0 and 7.0) on dehydroascorbate reductase activity. Purified recombinant protein of MT-I and MT-II were with (A) or without (B) 4 mM glutathione in the reaction mixtures. The reaction was carried out at 30°C by adding 100 μL MT-I and MT-II solution (100 μg protein, 100 mM phosphate buffer, pH 7.0 and 6.0) to 0.9 mL DHA solution with or without 4 mM glutathione. The increase of absorbance at 265 nm was recorded for 5 min. The experiments were done twice and a representative one is shown.

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