Table 1 Genomic sequences flanking COKC insertion in transgenic rice plants
Line | Chromosome/BACs | Insertion position (bp) | GenBank accession no. | Identities | Primers | |
|---|---|---|---|---|---|---|
COKC specific | Site specific | |||||
K19-H3 | 8/OSJNBa0095C12 | 46381 | AY360385 | 231/231 (100%) | GGTATTTCTTACATGGGCTG | GCCTTTTGACATGTAGCG |
GTCATACATTCTGATCATGAG | ||||||
K19-C2 | 2/OSJNBa0091C16 | 141691 | AP005820 | 198/200 (99%) | GGTATTTCTTACATGGGCTG | GCCGGTGGAGTGTCCTCCG |
GGTCGGCCTTGTTCGAGCG | ||||||
K19-H5 | 10/OSJNBa0094J09 | 56754 | AC078839 | 89/89 (100%) | GGTATTTCTTACATGGGCTG | GCCATAGCAAAGATCAGATC |
GAAATCTATTGCGTGCCAG | ||||||
K17-H3 | 7/P0045F02 | 30502 | AP004268 | 344/344 (100%) | GGTATTTCTTACATGGGCTG | CATAAGTTGTAGCAAAGCATAC |
ATTCATTTCATCTCTCAATTA | ||||||
K20-H6 | 1/OSJNOa264G09 | 14881 | AP008219 | 256/256 (100%) | GGTATTTCTTACATGGGCTG | GCTGAAGACATTCAGAGGGAG |
CTGCAGAAAATAAGCATATT | ||||||
K24-C2 | 12/OSJNBa0037B01 | 58801 | AL928751 | 155/155 (100%) | GGTATTTCTTACATGGGCTG | CTGATGAGCAAGAGAAAAAG |
CCGCCTCCTGGTCAGCTC | ||||||