Correlation between DNA methylation and chronological age of Moso bamboo (Phyllostachys heterocycla var. pubescens)

Botanical Studies

Table 2 DNA methylation levels in Moso bamboo at different chronological ages

Source of variation	Total rate of methylation (%)						Mean	Variance test
Age	E3HM2	E3HM6	E3HM7	E4HM5	E4HM6	E5HM5
2	14.28	15.38	10.63	17.46	19.4	32.5	18.28 ± 7.58^a*	SS=0.263, df=8, MS=0.033, F=14.231, Sig=0.000, P<0.01
6	14.28	13.72	15.38	24.24	23.19	32.91	20.62 ± 7.56^a
7	12	14.28	21.56	20.31	24.24	30.49	20.48 ± 6.72^a
13	14.28	20.41	10.71	20.28	22.39	37.35	20.90 ± 9.18^ab
18	18.18	26.41	23.64	23.08	30.77	39.24	26.89 ± 7.33^bc
32	25	31.48	25.45	27.69	28.36	44.3	30.38 ± 7.20^bc
34	23.07	25.92	28.84	26.56	29.23	36.25	28.31 ± 4.48^bc
44	20.83	25	31.48	32.81	31.81	36.25	29.70 ± 5.67^bc
63	26.66	50	37.04	32.81	30.76	50	37.88 ± 9.97^cd

Total rate of methylation (%) = Total number of methylation bands/Total number of amplified bands × 100%; SS represents Stdev square, df represents degree freedom, MS represents Mean square.
*Values followed by different small letters indicate L.S.D significant difference at p<0.05.