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Botanical Studies

Table 2 Influence of container closure type on induction of multiple shoots in G. tenuifolia

From: In vitro propagation and analysis of secondary metabolites in Glossogyne tenuifolia (Hsiang-Ju) - a medicinal plant native to Taiwan

Ventilation closure*

Culture medium

Explants induced multiple shoots (%)***

Average No. of shoots / explant ***

Fresh weight / shoot (mg) ***

AF2

½ X MS basal

61.4 ± 6.2 b

2.3 ± 0.2 c

128.7 ± 6.8 c

AF2

NAA (0.1) + BA (0.1)

100.0 ± 0.0 a

9.7 ± 0.4 a

472.1 ± 15.2 a

DP4 **

½ X MS basal

68.6 ± 2.5 b

2.5 ± 0.1 c

73.5 ± 2.8 d

DP4

NAA (0.1) + BA (0.1)

100.0 ± 0.0 a

7.1 ± 0.5 b

326.2 ± 12.2 b

  1. *Basal medium:½ X MS basal salts supplemented with 3% sucrose and 0.9% Difco Bacto-agar. Concentrations of PGRs in the parentheses represent mg/L values. Observations were recorded after 35 days of culture.
  2. ** AF2 = Culture container closed with 2 layers of Aluminum foil;
  3. DP4 = Culture container sealed with 4 dispense papers. Culture containers were initially sealed with 4 dispense paper and parafilm layers. After 2 weeks, parafilm layer was removed to facilitate ventilation.
  4. ***Means followed by the same letter are not significantly different at 5% level by LSD (least significant difference) test.
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