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Table 2 Influence of container closure type on induction of multiple shoots in G. tenuifolia

From: In vitro propagation and analysis of secondary metabolites in Glossogyne tenuifolia (Hsiang-Ju) - a medicinal plant native to Taiwan

Ventilation closure* Culture medium Explants induced multiple shoots (%)*** Average No. of shoots / explant *** Fresh weight / shoot (mg) ***
AF2 ½ X MS basal 61.4 ± 6.2 b 2.3 ± 0.2 c 128.7 ± 6.8 c
AF2 NAA (0.1) + BA (0.1) 100.0 ± 0.0 a 9.7 ± 0.4 a 472.1 ± 15.2 a
DP4 ** ½ X MS basal 68.6 ± 2.5 b 2.5 ± 0.1 c 73.5 ± 2.8 d
DP4 NAA (0.1) + BA (0.1) 100.0 ± 0.0 a 7.1 ± 0.5 b 326.2 ± 12.2 b
  1. *Basal medium:½ X MS basal salts supplemented with 3% sucrose and 0.9% Difco Bacto-agar. Concentrations of PGRs in the parentheses represent mg/L values. Observations were recorded after 35 days of culture.
  2. ** AF2 = Culture container closed with 2 layers of Aluminum foil;
  3. DP4 = Culture container sealed with 4 dispense papers. Culture containers were initially sealed with 4 dispense paper and parafilm layers. After 2 weeks, parafilm layer was removed to facilitate ventilation.
  4. ***Means followed by the same letter are not significantly different at 5% level by LSD (least significant difference) test.