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Botanical Studies

Table 2 Induction of multiple shoots in shoot apices of Gentiana scabra Bunge

From: In vitro propagation of Gentiana scabra Bunge – an important medicinal plant in the Chinese system of medicines

CytokininX(mg/L−1)

No. of shootsY

Shoot lengthY(cm)

Fresh weight of rooted shootY(g)

BA

0

5.6 ± 0.8 c

4.82 ± 0.49 a

0.50 ± 0.15 d

0.1

7.1 ± 1.0 b

2.82 ± 0.27 d

0.55 ± 0.08 bcd

0.5

7.4 ± 1.4 b

2.43 ± 0.24 e

0.55 ± 0.17 bcd

1.0

8.8 ± 2.8 a

2.23 ± 0.43 e

0.53 ± 0.10 cd

2.0

9.1 ± 1.8 a

1.92 ± 0.42 f

0.53 ± 0.17 cd

Kin

0.1

5.5 ± 0.9 c

3.56 ± 0.31 b

0.59 ± 0.07 abc

0.5

6.0 ± 0.8 c

3.31 ± 0.17 c

0.60 ± 0.09 ab

1.0

5.7 ± 1.1 c

3.13 ± 0.26 c

0.60 ± 0.15 a

  1. – Influence of BA and Kin.
  2. XMedium – half strength MSBM, 3% sucrose, 0.9% agar, incubation period 6 weeks.
  3. YMeans followed by the same letter of a column are not significantly different.
  4. at 5% probability (p = 0.05) level by least significant difference (LSD) test.
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