Subcellular localization of mBFP or mBFP-mS
C fusion protein in protoplasts of transgenic Arabidopsis. Five different transgenic Arabidopsis lines in which the expression of mBFP or mBFP-mS1C gene was driven by RbcS promoter were generated by floral dip method, and the expressed mBFP or mBFP-mS1C fusion proteins were expected to accumulate in the cytosol (CY, pBin-R-Cy-mBFP), ER (mBFP, pBin-R-Er-mBFP; mBFP-mS1C, pBin-R-Er-mBFP-mS1C), chloroplast (CP, pBin-R-Cp-mBFP) or mitochondria (MT, pBin-R-Mt-mBFP), respectively. The protoplasts were isolated from the leaf tissues of transgenic Arabidopsis lines and wild type (WT), and then were imaged under confocal microscope. Alternatively, the protoplasts isolated from transgenic pBin-R-Mt-mBFP line were treated with MitoTracker (Invitrogen) before imaging. BL, bright light; Red, chlorophyll auto-fluorescence; Blue, mBFP blue fluorescence; Green, green fluorescence of MitoTracker. The magnification is 1,000 X and the ruler shown is 20 μm in length.