Cellular lipid content of Nannochloropsis salina . The lipid contents were monitored (A) after 2, 8 and 12 days of incubation under optimal conditions and in response to three depleted nitrogen concentrations (B) after 2, 6 and 10 days of incubation in response to five different temperature degrees. The error bars indicate mean ± SE (n = 3). The effects of the treatments were tested by one-way analysis of variance (ANOVA). Means were compared between the treatments using the LSD (least significant difference) test at the 0.05 probability level.