Triglyceride content of Nannochloropsis salina . The triglyceride contents were measured (A) after 2, 8 and 12 days of cultivation under optimal conditions and three depleted nitrogen concentrations (B) after 2, 6 and 10 days of cultivation under five different temperature degrees. The error bars indicate mean ± SE (n = 3). The effects of the treatments were tested by one-way analysis of variance (ANOVA). Means were compared between the treatments using the LSD (least significant difference) test at the 0.05 probability level.