Fig. 1

The alignment of the deduced amino acid sequences of SaSTT3A and SaSTT3B with their homologs. The multiple alignments among SaSTT3A (a) or SaSTT3B (b) with their homologs were built by ClustalW2 server (http://www.ebi.ac.uk/Tools/msa/clustalw2/). Identical residues ≥4 sequences are shaded black, while similar residues were shaded in gray using the Boxshade server at http://www.ch.embnet.org/software/BOX_form.html. The conserved STT3 domain was boxed and the characteristic motif of WWDYG was marked with asterisks at the top of the alignment block. The accession number of the selected STT3 proteins are indicated as follows: Sb, Sorghum bicolor, STT3A: XP_002441399.1, STT3B: XP_002448765.1; Zm, Zea mays, STT3A: AFW78839.1, STT3B: NP_001131374.1; Os, Oryza sativa, STT3A: NP_001056068.1, STT3B: NP_001054248.1; Bd, Brachypodium distachyon, STT3A: XP_003568077.1, STT3B: XP_003580816.1; At, Arabidopsis thaliana, STT3A: NP_568380.1, STT3B: NP_174675.2; Sc, Saccharomyces cerevisiae, STT3: CAA96722.1