Fig. 5

The detection of SaSTT3A and SaSTT3B expression in Arabidopsis stt3a-2 mutant. The transgenic plants expressing SaSTT3A or SaSTT3B gene were confirmed using RT-PCR and real-time PCR method. One microgram of total RNA extracted from WT, stt3a-2, vector control, 35S:SaSTT3A/stt3a-2 or 35S:SaSTT3B/stt3a-2 seedlings which were grown in the soil for 4 weeks, were used for reverse transcription. a, b RT-PCR and real-time PCR analysis of 35S:SaSTT3A transgenic lines. c, d RT-PCR and real-time PCR analysis of 35S:SaSTT3B transgenic lines