Skip to main content

Table 1 Influence of abscisic acid (ABA) on induction of somatic embryos in callus of P. japonicum

From: Influence of LED light spectra on in vitro somatic embryogenesis and LC–MS analysis of chlorogenic acid and rutin in Peucedanum japonicum Thunb.: a medicinal herb

ABA (mg/L) Percentage of callus clumps showing somatic embryo Total number of somatic embryos Average callus fresh weight/clump (mg)a
0 15 10 (t) 447 ± 48 a
0.5 15 27 (g) 336 ± 16 b
1.0 20 44 (g) 329 ± 42 b
2.0 25 17 (g) 212 ± 27 c
4.0 30 13 (g) 172 ± 12 d
  1. Culture medium: MS basal medium + 3 % sucrose + 0.4 % GPP. Total number of callus clumps = 20 (10 in each petridish); each callus clump = 100 mg; Observations at 60 days of culture
  2. g globular, t torpedo
  3. aMean ± standard error. Means within each column followed by the same letter(s) are not significantly different at 5 % level by Fisher's protected LSD test