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Botanical Studies

Table 1 Influence of abscisic acid (ABA) on induction of somatic embryos in callus of P. japonicum

From: Influence of LED light spectra on in vitro somatic embryogenesis and LC–MS analysis of chlorogenic acid and rutin in Peucedanum japonicum Thunb.: a medicinal herb

ABA (mg/L)

Percentage of callus clumps showing somatic embryo

Total number of somatic embryos

Average callus fresh weight/clump (mg)a

0

15

10 (t)

447 ± 48 a

0.5

15

27 (g)

336 ± 16 b

1.0

20

44 (g)

329 ± 42 b

2.0

25

17 (g)

212 ± 27 c

4.0

30

13 (g)

172 ± 12 d

  1. Culture medium: MS basal medium + 3 % sucrose + 0.4 % GPP. Total number of callus clumps = 20 (10 in each petridish); each callus clump = 100 mg; Observations at 60 days of culture
  2. g globular, t torpedo
  3. aMean ± standard error. Means within each column followed by the same letter(s) are not significantly different at 5 % level by Fisher's protected LSD test
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