Illumina-based transcriptomic profiling of Panax notoginseng in response to arsenic stress

Botanical Studies

Table 2 DEGs between HAs and CK identified by illumina and validated by qRT-PCR

Gene ID	Illumina sequencing	QRT-PCR	KO description
comp139796_c2	−53.83^a	−138.82	chalcone synthase
comp78650_c0	−26.86	−80.31	flavonol synthase
comp129681_c0	−3.78	−9.73	hydroxymethyl glutaryl CoA synthase
comp106407_c0	−10.00	−25.62	3-hydroxy-3-methylglutaryl-coenzyme A reductase
comp140511_c0	−3.30	−5.73	Dammarenediol-II synthase
comp126977_c0	−6.04	−4.79	glycosyltransferase
comp141084_c0	−2.13	−6.34	glycosyltransferase
comp140248_c0	6.98^b	19.41	glutathione S-transferase
comp117631_c0	8.19	25.85	glutathione S-transferase
comp128853_c0	2.05	63.56	myb proto-oncogene protein, plant
comp128641_c0	2.32	2.17	myb proto-oncogene protein, plant
comp66279_c0	3.55	2.63	myb proto-oncogene protein, plant
comp128496_c0	5.93	8.83	myb proto-oncogene protein, plant
comp77739_c0	8.52	8.26	myb proto-oncogene protein, plant
comp109390_c0	6.71	5.20	heat shock transcription factor, other eukaryote
comp141438_c1	5.22	9.27	heat shock 90 kDa protein
comp142366_c1	6.33	4.68	heat shock 70 kDa protein
comp141020_c0	7.02	21.39	heat shock 70 kDa protein
comp134360_c1	12.85	9.71	heat shock 70 kDa protein
comp134360_c0	13.58	20.37	heat shock 70 kDa protein

HAs seedlings of Panax notoginseng stressed by arsenic (40 mg L⁻¹ Na₂HAsO₄·7H₂O) for 14 days. CK seedlings for control. DEGs differentially expressed genes
^aNegative values represent the folds of down-regulated expression of genes in HAs compared with CK
^bPositive values represent the folds of up-regulated expression of genes in HAs compared with CK