Fig. 1
From: Efficient and heritable transformation of Phalaenopsis orchids
Procedures for Agrobaterium-mediated transformation of protocorms in Phalaenopsis orchid. a Mature orchid capsule at 4 months after pollination. b Stage of mature seeds ready for sowing. c Growth of protocorms sown on 1/2 MS agar plates for 1 month. d Healthy protocorm ready for infection. e Agrobacteria infection. f The 1st round selection for hygromycin resistance on T2 media containing 25 ppm hygromycin and 40 ppm meropenem (T2 + MH). g The 2nd round selection for hygromycin resistance on T2 + MH. h Growth of explants on 1/2 MS plate. i Growth of transgenic lines in jar. j Transplanted transgenic orchid to 1.7″ pot containing sphagnum moss