Fig. 4From: The roles of Arabidopsis HSFA2, HSFA4a, and HSFA7a in the heat shock response and cytosolic protein responseAnalysis of tunicamycin (Tm) responsiveness of AtHSF mutants. a RT-PCR analysis of transcript levels in WT seedlings treated with Tm or solvent (dimethylformamide; DMF) and HS for the indicated times. mRNA expression of AtbZIP60 was a positive control for HS and Tm treatment. AtTubulin level was an internal control. Two biological repeats were performed, and similar results were obtained. b, c Relative GUS activity of seedlings treated with Tm. Data are mean ± SE GUS activity relative to that of non-stress control condition (Ctrl) from three independent experimentsBack to article page