Table 1 Oligonucleotides used in RT-PCR
Gene | Primer name | Sequence |
|---|---|---|
AtHSFA2 | AtHSFA2-Fw | 5′-CCATGGAAGAACTGAAAGTGGAAATGGAGG-3′ |
AtHSFA2-Rv | 5′-GCGGCCGCAGGTTCCGAACCAAG-3′ | |
AtHSFA4a | AtHSFA4a-Fw | 5′-CATCAAGTGGAACAGTTAGA-3′ |
AtHSFA4a-Rv | 5′-ACTCCGGCTTTATCTTTATC-3′ | |
AtHSFA5 | AtHSFA5-Fw | 5′-AGCAAGAGTGAATGATGTAT-3′ |
AtHSFA5-Rv | 5′-CTACTTACGCTTTTTCAGTC-3′ | |
AtHSFA7a | AtHSFA7a-Fw | 5′-ATCAAAGCTATGGAACAGAG-3′ |
AtHSFA7a-Rv | 5′-AACTCTCATCACTAAGCAAC-3′ | |
AtHSFB2a | AtHSFB2a-Fw | 5′-TTGAGACATTATAATCGAAC-3′ |
AtHSFB2a-Rv | 5′-TCTAAAAATGTACTTGTGAT-3′ | |
AtHSFB2b | AtHSFB2b Fw | 5′-GAGGAGAATAACTCCGGTAA-3′ |
AtHSFB2b Rv | 5′-ATGCAATGGGGATCAGTAAC-3′ | |
AtTubulin | AtTubulin Fw | 5′-GCCAATCCGGTGCTGGTAACA-3′ |
AtTubulin Rv | 5′-CATACCAGATCCAGTTCCTCCTCCC-3′ | |
AtbZIP60 | AtbZIP60-Fw | 5′-AGGACGTATGCTTGAGTGCTTCGT-3′ |
AtbZIP60-Rv | 5′-TTCTGGACGTAGGAGGCAACACT-3′ | |
GUS | GUS-Fw | 5′-GGCCTGTGGGCATTCAGTCT-3′ |
GUS-Rv | 5′-AGTTCAGTTCGTTGTTCACACAA-3′ |