Table 1 Locations and known functions of ZIP proteins in Arabidopsis and rice

AtIRT1

At4g19690

∆fet3∆fet4 (Y); ∆zrt1∆zrt2 (Y); ∆ctr1 (N); ∆smf1 (Y)

Early endosome, vacuole, trans-Golgi network and cell membrane; root epidermis, flower

Increased Cd sensitivity of overexpression plant/yeast

Reduced Cd sensitivity of irt1

Inhibited IRT1-dependent Fe/Mn/Zn uptake by Cd in yeast

Reduced Cd uptake of irt1

Eide et al. (1996), Vert et al. (2002, 2001), Korshunova et al. (1999), Rogers et al. (2000), Connolly et al. (2002), Varotto et al. (2002), Henriques et al. (2002), Nishida et al. (2011), Shin et al. (2013), Potocki et al. (2013), Fan et al. (2014), Barberon et al. (2014), Blum et al. (2014)

AtIRT2

At4g19680

∆fet3∆fet4 (Y); ∆zrt1∆zrt2 (Y); ∆smf1 (N)

Intracellular vesicles; root epidermis

No altered Cd sensitivity of overexpression yeast

Increased Cd uptake and IRT1 expression of overexpression plant

Vert et al. (2001, 2009), Wintz et al. (2003), Varotto et al. (2002)

AtIRT3

At1g60960

∆Spzrt1 (Y); ∆zrt1∆zrt2 (Y); ∆fet3∆fet4 (Y); ∆smf1 (N)

Cell membrane; broadly expressed

No altered Cd sensitivity of overexpression yeast

Lin et al. (2009), Talke et al. (2006), Shanmugam et al. (2011), Hammes et al. (2005)

AtZIP1

At3g12750

∆zrt1∆zrt2 (Y); ∆fet3∆fet4 (N); ∆ctr1∆ctr3 (N); ∆smf1 (Y)

Vacuolar; predominantly root stele and leaf vasculature

Inhibited ZIP1-dependent Zn uptake by Cd in yeast, to a less extent

Grotz et al. (1998), Milner et al. (2013)

AtZIP2

At5g59520

∆zrt1∆zrt2 (Y); ∆fet3∆fet4 (N); ∆ctr1∆ctr3 (N); ∆smf1 (Y); ∆ctr1 (Y)

Cell membrane; predominantly mature root stele

Inhibited ZIP2-dependent Zn uptake by Cd in yeast

Grotz et al. (1998), Milner et al. (2013), Wintz et al. (2003)

AtZIP3

At2g32270

∆zrt1∆zrt2 (Y); ∆fet3∆fet4 (N); ∆ctr1∆ctr3 (N); ∆smf1 (N)

Predominantly root

Inhibited ZIP3-dependent Zn uptake by Cd in yeast, to a less extent

Grotz et al. (1998), Talke et al. (2006), Milner et al. (2013)

AtZIP4

At1g10970

∆zrt1∆zrt2 (N); ∆fet3∆fet4 (N); ∆ctr1 (Y)

Root and leaf

N/A

Grotz et al. (1998), Talke et al. (2006), Wintz et al. (2003)

AtZIP5

At1g05300

∆zrt1∆zrt2 (N); ∆fet3∆fet4 (N); ∆ctr1∆ctr3 (N); ∆smf1 (Y)

Root

N/A

Milner et al. (2013), Wintz et al. (2003)

AtZIP6

At2g30080

∆zrt1∆zrt2 (N); ∆fet3∆fet4 (N); ∆ctr1∆ctr3 (N); ∆smf1 (Y)

Root

N/A

Milner et al. (2013), Hammes et al. (2005)

AtZIP7

At2g04032

∆zrt1∆zrt2 (Y); ∆fet3∆fet4 (Y); ∆ctr1∆ctr3 (N); ∆smf1 (Y)

N/A

N/A

Milner et al. (2013)

AtZIP8

At5g45105

∆zrt1∆zrt2 (N); ∆fet3∆fet4 (N); ∆ctr1∆ctr3 (N); ∆smf1 (N)

N/A

N/A

Milner et al. (2013)

AtZIP9

At4g33020

∆zrt1∆zrt2 (N); ∆fet3∆fet4 (N); ∆ctr1∆ctr3 (N); ∆smf1 (Y)

Root and shoot

N/A

Talke et al. (2006), Milner et al. (2013), Wintz et al. (2003), Inaba et al. (2015)

AtZIP10

At1g31260

∆zrt1∆zrt2 (Y); ∆fet3∆fet4 (N); ∆ctr1∆ctr3 (N); ∆smf1 (N)

N/A

N/A

Milner et al. (2013)

AtZIP11

At1g55910

∆zrt1∆zrt2 (Y); ∆fet3∆fet4 (N); ∆ctr1∆ctr3 (N); ∆smf1 (N)

N/A

N/A

Milner et al. (2013)

AtZIP12

At5g62160

∆zrt1∆zrt2 (Y); ∆fet3∆fet4 (N); ∆ctr1∆ctr3 (N); ∆smf1 (N)

Root

N/A

Milner et al. (2013), Inaba et al. (2015)

OsIRT1

LOC_Os03g46470

∆fet3∆fet4 (Y); ∆ctr1 (N); ∆zrt1∆zrt2 (N); ∆smf1 (N); ∆frt1∆fet4∆fre1(Y); ∆frt1∆fet1∆fre3(Y)

Cell membrane; mainly in root epidermis (the inner layer of the cortex, and the stele) and stems (companion cells)

Increased Cd sensitivity of overexpression plant

Increased Cd sensitivity and Cd uptake of overexpression yeast

Ishimaru et al. (2006), Bughio et al. (2002), Lee and An (2009), Nakanishi et al. (2006), Ishimaru et al. (2005)

OsIRT2

LOC_Os03g46454

∆fet3∆fet4 (Y); ∆ctr1 (N); ∆zrt1∆zrt2 (N); ∆smf1 (N)

Cell membrane

Increased Cd sensitivity and Cd uptake of overexpression yeast

Ishimaru et al. (2006), Nakanishi et al. (2006)

OsZIP1

LOC_Os01g74110

∆zrt1∆zrt2 (Y); ∆smf1 (Y); ∆fet3∆fet4 (N)

Broadly expressed

Increased Cd sensitivity of overexpression yeast

Inhibited ZIP1-dependent Zn uptake by Cd in yeast

Ramesh et al. (2003, Ishimaru et al. (2005), Chen et al. (2008)

OsZIP2

LOC_Os03g29850

∆zrt1∆zrt2 (N);

N/A

No altered Cd sensitivity of overexpression yeast

Ramesh et al. (2003)

OsZIP3

LOC_Os04g52310

∆zrt1∆zrt2 (Y); ∆smf1 (Y); ∆fet3∆fet4 (N)

Mainly induced by zinc deficiency to higher levels in roots

No altered Cd sensitivity of overexpression yeast

Mildly increased ZIP3-dependent Zn uptake by Cd in yeast

Ramesh et al. (2003, Ishimaru et al. (2005), Chen et al. (2008)

OsZIP4

LOC_Os08g10630

∆zrt1∆zrt2 (Y); ∆frt1∆fet1∆fre3(N)

Cell membrane; phloem cells of leaves, roots and meristem

N/A

Ishimaru et al. (2005), Chen et al. (2008)

OsZIP5

LOC_Os05g39560

∆zrt1∆zrt2 (Y)

Cell membrane; mainly panicle

N/A

Chen et al. (2008), Lee et al. (2010a)

OsZIP6

LOC_Os05g07210

N/A

Root, shoot and panicle

Increased Cd uptake of overexpression cells

Chen et al. (2008)

OsZIP7

LOC_Os05g10940

N/A

Root, shoot and panicle

N/A

Chen et al. (2008), Yang et al. (2009)

OsZIP8

LOC_Os07g12890

N/A

Cell membrane; mainly root and panicle

N/A

Chen et al. (2008), Lee et al. (2010b), Yang et al. (2009)

OsZIP9

LOC_Os05g39540

N/A

Root, shoot and panicle

N/A

Chen et al. (2008)

OsZIP10

LOC_Os06g37010

N/A

N/A

N/A

N/A