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Table 1 Representative phosphorylated proteins identified by MALDI-TOF/TOF-MS and Pro-Q staining

From: Identification of phosphorylation proteins in response to water deficit during wheat flag leaf and grain development

Spot no.Protein nameProtein scorePeptide countp-valueSubcellular localizationProtein levelPhosphorylation level
Photosynthesis
L1Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit300180.021ChloroplastDownDown
L4Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit1120300.03ChloroplastDownDown
L5Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit695290.024ChloroplastUpUp
L8Chlorophyll a–b binding protein 8, chloroplastic11150.041ChloroplastUpUp
L9Chlorophyll a–b binding protein, chloroplastic399100.028ChloroplastUpUp
L10Ribulose bisphosphate carboxylase/oxygenase activase A, partial363170.029ChloroplastDownDown−
L11Ribulose bisphosphate carboxylase/oxygenase activase A, partial402170.042ChloroplastDownDown−
L14RuBisCO large subunit-binding protein subunit beta1030200.013ChloroplastDownDown
G1Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial166100.038ChloroplastUpDown
Energy metabolism
L13Ribulose bisphosphate carboxylase activase B539180.031ChloroplastUpUp
L16Fructose-bisphosphate aldolase, cytoplasmic isozyme 1606240.019CytoplasmUpUp
L17Chloroplast fructose-1,6-biphosphate aldolase490170.035ChloroplastUpUp
L19ATP synthase subunit255200.043ChloroplastDownDown
L21Phosphoglycerate kinase, chloroplastic916170.028ChloroplastUpUp
L25V-type proton ATPase subunit B 1538360.019ChloroplastUpUp
L26ATP-dependent Clp protease ATP-binding subunit clpA-like protein CD4B383380.02ChloroplastDownUp
G2Fructose-bisphosphate aldolase cytoplasmic isozyme118120.019CytoplasmUpUp
G4Triosephosphate-isomerase61930.018CytoplasmUpUp
G5Glyceraldehyde-3-phosphate dehydrogenase75140.032CytoplasmDownUp
G6Enolase366180.018CytoplasmDownDown
G7Pyruvate, phosphate dikinase 1491390.018ChloroplastUpUp
G8Dihydrolipoyl dehydrogenase253230.015MitochondrionUpUp
G9Aconitate hydratase163250.043CytoplasmUpUp
Storage substance biosynthesis
G10Sucrose synthase type 2809310.002CytoplasmUpUp
G11Sucrose synthase 2432350.028CytoplasmUpUp
G12Phosphoglucomutase476180.019CytoplasmUpUp
Stress defense
G13Heat shock protein 101916400.039NucleusUpUp
G1417.9 kDa class I heat shock protein-like224210.017CytoplasmUpUp
G15Oxalate oxidase 29750.021Cell-MembraneUpUp
G16Dehydroascorbate reductase812150.035CytoplasmUpDown
G17Disease resistance protein RPP848140.022CytoplasmicUpUp
G18Peroxidase 1340130.032VacuoleDownUp
G19Translationally-controlled tumor protein552160.022CytoplasmDownUp+
G31Hypothetical protein TRIUR3_2126063280.006CytoplasmDownDown
G35Cold regulated protein21060.021CytoplasmDownDown
G39Translationally controlled tumor protein26470.021CytoplasmUpUp
Carbon metabolism
L20Arabinoxylan arabinofuranohydrolase isoenzyme AXAH-II251120.029Cell-MembraneUpUp
L24Predicted protein316140.039ChloroplastDownUp
G24Basic endochitinase C13030.029ExtracellularUpDown
G28Formate dehydrogenase19630.026MitochondrionUpUp
G30Glucose and ribitol Dehydrogenase-like protein264110.032CytoplasmUpDown
Protein biosynthesis and degradation
L18Peptidyl-prolylcis-trans isomerase CYP38371210.015ChloroplastDownDown
L22Methionine synthase 1 enzyme391270.032CytoplasmDownUp
L23Methionine synthase 1 enzyme391270.006CytoplasmDownUp
G20Globulin-1 S allele416160.024VacuoleUpUp
G22Serpin-Z2B712180.034ChloroplastDownDown
G23ATP-dependent zinc metalloprotease FTSH 253120.027ChloroplastUpUp
G25Alanine aminotransferase 2304220.017CytoplasmDownUp
G27Serpin 1500170.041ExtracellularUpDown
G32Putative methionine synthase15710.014CytoplasmUpUp
G33Aspartate aminotransferase19490.021CytoplasmDownUp
G34Globulin 3489200.017VacuoleDownUp
G37Elongation factor 1-beta15940.042VacuoleDownDown
  1. Up: upregulated in phosphorylation level; Down: downregulated in phosphorylation level. Up+: the phosphoproteins were only identified in drought treatment. Down−: the phosphoproteins were only identified in CK