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Table 1 Representative phosphorylated proteins identified by MALDI-TOF/TOF-MS and Pro-Q staining

From: Identification of phosphorylation proteins in response to water deficit during wheat flag leaf and grain development

Spot no.

Protein name

Protein score

Peptide count

p-value

Subcellular localization

Protein level

Phosphorylation level

Photosynthesis

L1

Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit

300

18

0.021

Chloroplast

Down

Down

L4

Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit

1120

30

0.03

Chloroplast

Down

Down

L5

Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit

695

29

0.024

Chloroplast

Up

Up

L8

Chlorophyll a–b binding protein 8, chloroplastic

111

5

0.041

Chloroplast

Up

Up

L9

Chlorophyll a–b binding protein, chloroplastic

399

10

0.028

Chloroplast

Up

Up

L10

Ribulose bisphosphate carboxylase/oxygenase activase A, partial

363

17

0.029

Chloroplast

Down

Down−

L11

Ribulose bisphosphate carboxylase/oxygenase activase A, partial

402

17

0.042

Chloroplast

Down

Down−

L14

RuBisCO large subunit-binding protein subunit beta

1030

20

0.013

Chloroplast

Down

Down

G1

Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial

166

10

0.038

Chloroplast

Up

Down

Energy metabolism

L13

Ribulose bisphosphate carboxylase activase B

539

18

0.031

Chloroplast

Up

Up

L16

Fructose-bisphosphate aldolase, cytoplasmic isozyme 1

606

24

0.019

Cytoplasm

Up

Up

L17

Chloroplast fructose-1,6-biphosphate aldolase

490

17

0.035

Chloroplast

Up

Up

L19

ATP synthase subunit

255

20

0.043

Chloroplast

Down

Down

L21

Phosphoglycerate kinase, chloroplastic

916

17

0.028

Chloroplast

Up

Up

L25

V-type proton ATPase subunit B 1

538

36

0.019

Chloroplast

Up

Up

L26

ATP-dependent Clp protease ATP-binding subunit clpA-like protein CD4B

383

38

0.02

Chloroplast

Down

Up

G2

Fructose-bisphosphate aldolase cytoplasmic isozyme

118

12

0.019

Cytoplasm

Up

Up

G4

Triosephosphate-isomerase

619

3

0.018

Cytoplasm

Up

Up

G5

Glyceraldehyde-3-phosphate dehydrogenase

75

14

0.032

Cytoplasm

Down

Up

G6

Enolase

366

18

0.018

Cytoplasm

Down

Down

G7

Pyruvate, phosphate dikinase 1

491

39

0.018

Chloroplast

Up

Up

G8

Dihydrolipoyl dehydrogenase

253

23

0.015

Mitochondrion

Up

Up

G9

Aconitate hydratase

163

25

0.043

Cytoplasm

Up

Up

Storage substance biosynthesis

G10

Sucrose synthase type 2

809

31

0.002

Cytoplasm

Up

Up

G11

Sucrose synthase 2

432

35

0.028

Cytoplasm

Up

Up

G12

Phosphoglucomutase

476

18

0.019

Cytoplasm

Up

Up

Stress defense

G13

Heat shock protein 101

916

40

0.039

Nucleus

Up

Up

G14

17.9 kDa class I heat shock protein-like

224

21

0.017

Cytoplasm

Up

Up

G15

Oxalate oxidase 2

97

5

0.021

Cell-Membrane

Up

Up

G16

Dehydroascorbate reductase

812

15

0.035

Cytoplasm

Up

Down

G17

Disease resistance protein RPP8

48

14

0.022

Cytoplasmic

Up

Up

G18

Peroxidase 1

340

13

0.032

Vacuole

Down

Up

G19

Translationally-controlled tumor protein

552

16

0.022

Cytoplasm

Down

Up+

G31

Hypothetical protein TRIUR3_21260

632

8

0.006

Cytoplasm

Down

Down

G35

Cold regulated protein

210

6

0.021

Cytoplasm

Down

Down

G39

Translationally controlled tumor protein

264

7

0.021

Cytoplasm

Up

Up

Carbon metabolism

L20

Arabinoxylan arabinofuranohydrolase isoenzyme AXAH-II

251

12

0.029

Cell-Membrane

Up

Up

L24

Predicted protein

316

14

0.039

Chloroplast

Down

Up

G24

Basic endochitinase C

130

3

0.029

Extracellular

Up

Down

G28

Formate dehydrogenase

196

3

0.026

Mitochondrion

Up

Up

G30

Glucose and ribitol Dehydrogenase-like protein

264

11

0.032

Cytoplasm

Up

Down

Protein biosynthesis and degradation

L18

Peptidyl-prolylcis-trans isomerase CYP38

371

21

0.015

Chloroplast

Down

Down

L22

Methionine synthase 1 enzyme

391

27

0.032

Cytoplasm

Down

Up

L23

Methionine synthase 1 enzyme

391

27

0.006

Cytoplasm

Down

Up

G20

Globulin-1 S allele

416

16

0.024

Vacuole

Up

Up

G22

Serpin-Z2B

712

18

0.034

Chloroplast

Down

Down

G23

ATP-dependent zinc metalloprotease FTSH 2

53

12

0.027

Chloroplast

Up

Up

G25

Alanine aminotransferase 2

304

22

0.017

Cytoplasm

Down

Up

G27

Serpin 1

500

17

0.041

Extracellular

Up

Down

G32

Putative methionine synthase

157

1

0.014

Cytoplasm

Up

Up

G33

Aspartate aminotransferase

194

9

0.021

Cytoplasm

Down

Up

G34

Globulin 3

489

20

0.017

Vacuole

Down

Up

G37

Elongation factor 1-beta

159

4

0.042

Vacuole

Down

Down

  1. Up: upregulated in phosphorylation level; Down: downregulated in phosphorylation level. Up+: the phosphoproteins were only identified in drought treatment. Down−: the phosphoproteins were only identified in CK