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Table 1 Representative phosphorylated proteins identified by MALDI-TOF/TOF-MS and Pro-Q staining

From: Identification of phosphorylation proteins in response to water deficit during wheat flag leaf and grain development

Spot no. Protein name Protein score Peptide count p-value Subcellular localization Protein level Phosphorylation level
Photosynthesis
L1 Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit 300 18 0.021 Chloroplast Down Down
L4 Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit 1120 30 0.03 Chloroplast Down Down
L5 Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit 695 29 0.024 Chloroplast Up Up
L8 Chlorophyll a–b binding protein 8, chloroplastic 111 5 0.041 Chloroplast Up Up
L9 Chlorophyll a–b binding protein, chloroplastic 399 10 0.028 Chloroplast Up Up
L10 Ribulose bisphosphate carboxylase/oxygenase activase A, partial 363 17 0.029 Chloroplast Down Down−
L11 Ribulose bisphosphate carboxylase/oxygenase activase A, partial 402 17 0.042 Chloroplast Down Down−
L14 RuBisCO large subunit-binding protein subunit beta 1030 20 0.013 Chloroplast Down Down
G1 Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial 166 10 0.038 Chloroplast Up Down
Energy metabolism
L13 Ribulose bisphosphate carboxylase activase B 539 18 0.031 Chloroplast Up Up
L16 Fructose-bisphosphate aldolase, cytoplasmic isozyme 1 606 24 0.019 Cytoplasm Up Up
L17 Chloroplast fructose-1,6-biphosphate aldolase 490 17 0.035 Chloroplast Up Up
L19 ATP synthase subunit 255 20 0.043 Chloroplast Down Down
L21 Phosphoglycerate kinase, chloroplastic 916 17 0.028 Chloroplast Up Up
L25 V-type proton ATPase subunit B 1 538 36 0.019 Chloroplast Up Up
L26 ATP-dependent Clp protease ATP-binding subunit clpA-like protein CD4B 383 38 0.02 Chloroplast Down Up
G2 Fructose-bisphosphate aldolase cytoplasmic isozyme 118 12 0.019 Cytoplasm Up Up
G4 Triosephosphate-isomerase 619 3 0.018 Cytoplasm Up Up
G5 Glyceraldehyde-3-phosphate dehydrogenase 75 14 0.032 Cytoplasm Down Up
G6 Enolase 366 18 0.018 Cytoplasm Down Down
G7 Pyruvate, phosphate dikinase 1 491 39 0.018 Chloroplast Up Up
G8 Dihydrolipoyl dehydrogenase 253 23 0.015 Mitochondrion Up Up
G9 Aconitate hydratase 163 25 0.043 Cytoplasm Up Up
Storage substance biosynthesis
G10 Sucrose synthase type 2 809 31 0.002 Cytoplasm Up Up
G11 Sucrose synthase 2 432 35 0.028 Cytoplasm Up Up
G12 Phosphoglucomutase 476 18 0.019 Cytoplasm Up Up
Stress defense
G13 Heat shock protein 101 916 40 0.039 Nucleus Up Up
G14 17.9 kDa class I heat shock protein-like 224 21 0.017 Cytoplasm Up Up
G15 Oxalate oxidase 2 97 5 0.021 Cell-Membrane Up Up
G16 Dehydroascorbate reductase 812 15 0.035 Cytoplasm Up Down
G17 Disease resistance protein RPP8 48 14 0.022 Cytoplasmic Up Up
G18 Peroxidase 1 340 13 0.032 Vacuole Down Up
G19 Translationally-controlled tumor protein 552 16 0.022 Cytoplasm Down Up+
G31 Hypothetical protein TRIUR3_21260 632 8 0.006 Cytoplasm Down Down
G35 Cold regulated protein 210 6 0.021 Cytoplasm Down Down
G39 Translationally controlled tumor protein 264 7 0.021 Cytoplasm Up Up
Carbon metabolism
L20 Arabinoxylan arabinofuranohydrolase isoenzyme AXAH-II 251 12 0.029 Cell-Membrane Up Up
L24 Predicted protein 316 14 0.039 Chloroplast Down Up
G24 Basic endochitinase C 130 3 0.029 Extracellular Up Down
G28 Formate dehydrogenase 196 3 0.026 Mitochondrion Up Up
G30 Glucose and ribitol Dehydrogenase-like protein 264 11 0.032 Cytoplasm Up Down
Protein biosynthesis and degradation
L18 Peptidyl-prolylcis-trans isomerase CYP38 371 21 0.015 Chloroplast Down Down
L22 Methionine synthase 1 enzyme 391 27 0.032 Cytoplasm Down Up
L23 Methionine synthase 1 enzyme 391 27 0.006 Cytoplasm Down Up
G20 Globulin-1 S allele 416 16 0.024 Vacuole Up Up
G22 Serpin-Z2B 712 18 0.034 Chloroplast Down Down
G23 ATP-dependent zinc metalloprotease FTSH 2 53 12 0.027 Chloroplast Up Up
G25 Alanine aminotransferase 2 304 22 0.017 Cytoplasm Down Up
G27 Serpin 1 500 17 0.041 Extracellular Up Down
G32 Putative methionine synthase 157 1 0.014 Cytoplasm Up Up
G33 Aspartate aminotransferase 194 9 0.021 Cytoplasm Down Up
G34 Globulin 3 489 20 0.017 Vacuole Down Up
G37 Elongation factor 1-beta 159 4 0.042 Vacuole Down Down
  1. Up: upregulated in phosphorylation level; Down: downregulated in phosphorylation level. Up+: the phosphoproteins were only identified in drought treatment. Down−: the phosphoproteins were only identified in CK