Identification of phosphorylation proteins in response to water deficit during wheat flag leaf and grain development

Luo, Fei; Deng, Xiong; Liu, Yue; Yan, Yueming

doi:10.1186/s40529-018-0245-7

Botanical Studies

Table 1 Representative phosphorylated proteins identified by MALDI-TOF/TOF-MS and Pro-Q staining

From: Identification of phosphorylation proteins in response to water deficit during wheat flag leaf and grain development

Spot no.	Protein name	Protein score	Peptide count	p-value	Subcellular localization	Protein level	Phosphorylation level
Photosynthesis
L1	Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit	300	18	0.021	Chloroplast	Down	Down
L4	Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit	1120	30	0.03	Chloroplast	Down	Down
L5	Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit	695	29	0.024	Chloroplast	Up	Up
L8	Chlorophyll a–b binding protein 8, chloroplastic	111	5	0.041	Chloroplast	Up	Up
L9	Chlorophyll a–b binding protein, chloroplastic	399	10	0.028	Chloroplast	Up	Up
L10	Ribulose bisphosphate carboxylase/oxygenase activase A, partial	363	17	0.029	Chloroplast	Down	Down−
L11	Ribulose bisphosphate carboxylase/oxygenase activase A, partial	402	17	0.042	Chloroplast	Down	Down−
L14	RuBisCO large subunit-binding protein subunit beta	1030	20	0.013	Chloroplast	Down	Down
G1	Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, partial	166	10	0.038	Chloroplast	Up	Down
Energy metabolism
L13	Ribulose bisphosphate carboxylase activase B	539	18	0.031	Chloroplast	Up	Up
L16	Fructose-bisphosphate aldolase, cytoplasmic isozyme 1	606	24	0.019	Cytoplasm	Up	Up
L17	Chloroplast fructose-1,6-biphosphate aldolase	490	17	0.035	Chloroplast	Up	Up
L19	ATP synthase subunit	255	20	0.043	Chloroplast	Down	Down
L21	Phosphoglycerate kinase, chloroplastic	916	17	0.028	Chloroplast	Up	Up
L25	V-type proton ATPase subunit B 1	538	36	0.019	Chloroplast	Up	Up
L26	ATP-dependent Clp protease ATP-binding subunit clpA-like protein CD4B	383	38	0.02	Chloroplast	Down	Up
G2	Fructose-bisphosphate aldolase cytoplasmic isozyme	118	12	0.019	Cytoplasm	Up	Up
G4	Triosephosphate-isomerase	619	3	0.018	Cytoplasm	Up	Up
G5	Glyceraldehyde-3-phosphate dehydrogenase	75	14	0.032	Cytoplasm	Down	Up
G6	Enolase	366	18	0.018	Cytoplasm	Down	Down
G7	Pyruvate, phosphate dikinase 1	491	39	0.018	Chloroplast	Up	Up
G8	Dihydrolipoyl dehydrogenase	253	23	0.015	Mitochondrion	Up	Up
G9	Aconitate hydratase	163	25	0.043	Cytoplasm	Up	Up
Storage substance biosynthesis
G10	Sucrose synthase type 2	809	31	0.002	Cytoplasm	Up	Up
G11	Sucrose synthase 2	432	35	0.028	Cytoplasm	Up	Up
G12	Phosphoglucomutase	476	18	0.019	Cytoplasm	Up	Up
Stress defense
G13	Heat shock protein 101	916	40	0.039	Nucleus	Up	Up
G14	17.9 kDa class I heat shock protein-like	224	21	0.017	Cytoplasm	Up	Up
G15	Oxalate oxidase 2	97	5	0.021	Cell-Membrane	Up	Up
G16	Dehydroascorbate reductase	812	15	0.035	Cytoplasm	Up	Down
G17	Disease resistance protein RPP8	48	14	0.022	Cytoplasmic	Up	Up
G18	Peroxidase 1	340	13	0.032	Vacuole	Down	Up
G19	Translationally-controlled tumor protein	552	16	0.022	Cytoplasm	Down	Up+
G31	Hypothetical protein TRIUR3_21260	632	8	0.006	Cytoplasm	Down	Down
G35	Cold regulated protein	210	6	0.021	Cytoplasm	Down	Down
G39	Translationally controlled tumor protein	264	7	0.021	Cytoplasm	Up	Up
Carbon metabolism
L20	Arabinoxylan arabinofuranohydrolase isoenzyme AXAH-II	251	12	0.029	Cell-Membrane	Up	Up
L24	Predicted protein	316	14	0.039	Chloroplast	Down	Up
G24	Basic endochitinase C	130	3	0.029	Extracellular	Up	Down
G28	Formate dehydrogenase	196	3	0.026	Mitochondrion	Up	Up
G30	Glucose and ribitol Dehydrogenase-like protein	264	11	0.032	Cytoplasm	Up	Down
Protein biosynthesis and degradation
L18	Peptidyl-prolylcis-trans isomerase CYP38	371	21	0.015	Chloroplast	Down	Down
L22	Methionine synthase 1 enzyme	391	27	0.032	Cytoplasm	Down	Up
L23	Methionine synthase 1 enzyme	391	27	0.006	Cytoplasm	Down	Up
G20	Globulin-1 S allele	416	16	0.024	Vacuole	Up	Up
G22	Serpin-Z2B	712	18	0.034	Chloroplast	Down	Down
G23	ATP-dependent zinc metalloprotease FTSH 2	53	12	0.027	Chloroplast	Up	Up
G25	Alanine aminotransferase 2	304	22	0.017	Cytoplasm	Down	Up
G27	Serpin 1	500	17	0.041	Extracellular	Up	Down
G32	Putative methionine synthase	157	1	0.014	Cytoplasm	Up	Up
G33	Aspartate aminotransferase	194	9	0.021	Cytoplasm	Down	Up
G34	Globulin 3	489	20	0.017	Vacuole	Down	Up
G37	Elongation factor 1-beta	159	4	0.042	Vacuole	Down	Down

Up: upregulated in phosphorylation level; Down: downregulated in phosphorylation level. Up+: the phosphoproteins were only identified in drought treatment. Down−: the phosphoproteins were only identified in CK

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