In vitro propagation of bulblets and LC–MS/MS analysis of isosteroidal alkaloids in tissue culture derived materials of Chinese medicinal herb Fritillaria cirrhosa D. Don

Botanical Studies

Table 3 Effects of different salt and sucrose concentrations on growth parameters of 1 month old in vitro seedlings of F. cirrhosa

MSBM strength^a	Sucrose conc. (%)	No. of seedlings inoculated	Survival (%)	Seedlings with bulblet formation (%)	Seedlings with callus formation (%)
1/2 X	0.5	43	72.1	67.4	4.7
1/2 X	1	51	52.9	52.9	0.0
1/2 X	1.5	25	60.0	44.0	24.0
1/2 X	2	47	72.3	68.1	12.8
1/2 X	2.5	35	91.4	91.4	17.1
1/2 X	3	24	75.0	75.0	4.2
1 X	0.5	30	56.7	53.3	6.7
1 X	1	39	69.2	69.2	7.7
1 X	1.5	41	56.1	56.1	12.2
1 X	2	29	34.5	31.0	6.9
1 X	2.5	32	65.6	62.5	6.3
1 X	3	89	51.7	49.4	11.24

^aMS salts and vitamins (MSBM) were supplemented with 0.4% GPP. Seedlings were incubated at 20 ℃. Data were recorded after 3 months of culture