Fig. 2
Variation in potyvirus P1/HC-Pro and recombined P1/HC-Pro plants. a Schematic diagram of P1 and HC-Pro amino acid sequence similarity among fifty-seven Potyviruses. The black boxes indicate conserved sequence regions. b The morphologic phenotype of P1/HCZy and P1/HCTe plants. Bar, 1 cm. c. Detection of P1 and HC-Pro in P1/HCZy, P1/HCTe, P1Zy/HCTu, and P1Te/HCTu plants. The @ symbol indicates CDC2 as an internal control. Two-asterisk (**) indicate cross-reaction of the α-HCZy antibodies. d Schematic of the binary plasmids containing the various recombined P1/HC-Pro constructs used in this study. e Phenotypes of various recombinant P1/HC-Pro transgenic plants. The photographs were taken of 3-week-old seedlings. Bar, 1 cm. f Heatmap of abnormal miRNA and miRNA* accumulation in various recombinant P1/HC-Pro plants. The values of heatmap were convert from miRNA northern blot. Significant upregulation (Student’s t test; P value < 0.05) is labeled in red. Gray indicates differential expression that is not significant. The values on the right indicate the results of the log2 (each sample/Col-0) formula