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Botanical Studies
Fig. 2 | Botanical Studies

Fig. 2

From: Purification, identification and characterization of Nag2 N-acetylglucosaminidase from Trichoderma virens strain mango

Fig. 2

Kinetic parameter and SDS-PAGE analysis of TvmNAG2, and protein sequence of TvNag2. A The activity of TvmNAG2 was determined using different concentrations of substrate, pNP-NAG. Michaelis–Menten and Lineweaver–Burk graphs were then plotted. B SDS-PAGE analysis: lane 1, the crude protein from the precipitation of 8-days T. virens filtrate with 80% (NH4)2SO4; lane 2, the affinity-purified TvmNAG2; M, protein marker. C TvmNAG2, lane 2 from B, was matched to T. virens Gv29-8 Nag2 (TvNag2, accession number, XM_014099474) after MALDI/MS analysis. The matched peptides of TvmNAG2 were marked in red

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