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Fig.5 | Botanical Studies

Fig.5

From: L-DOPA induces iron accumulation in roots of Ipomoea aquatica and Arabidopsis thaliana in a pH-dependent manner

Fig.5

Analysis of L-DOPA and its oxidation products and the influence of pH. A HPLC analysis of L-DOPA 10 min after solubilization in HCl at pH 2.3 and B in KOH at pH 8.7, corresponding to the two lowest pKa of L-DOPA. The experiment was carried out three times. C, D UHPLC-MS analysis of L-DOPA extracted from roots of Arabidopsis plants. Two compounds with very close elution time and m/z were detected. E SR-FTIR analysis of the dark precipitate forming in nutrient solution containing L-DOPA and of L-DOPA, melanin and DOPA-melanin. The precipitate has absorption properties similar to melanin and DOPA-melanin but distinct from them

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