Table 2 Effect of different media types and combinations and concentrations of PGRs on direct regeneration from anther culture in Bomby

M1

MS + 4 mg/L NAA + 1 mg/L BAP

1.46 ± 0.02^a

14.34 ± 0.24^e₂

2.35 ± 0.003^a

1.87 ± 0.003^a

M2

MS + 4 mg/L NAA + 0.1 mg/L BAP

0.83 ± 0.02^b

14.37 ± 0.28^e₂

1.87 ± 0.018^b

1.22 ± 0.003^b

M3

MS + 4 mg/L NAA + 0.5 mg/L BAP

0.64 ± 0.02^c

17.94 ± 0.11^c₂

1.58 ± 0.006^c

1.22 ± 0.003^b

M4

MS + 5 mg/L NAA + 0.1 mg/L BAP + 0.05 mg/L biotin

0.58 ± 0.02 ^cd

23.55 ± 0.16^a₂

1.22 ± 0.009^d

0.71 ± 0.00^c

M5

MS + 5 mg/L NAA + 1 mg/L BAP + 0.05 mg/L folic acid

0.41 ± 0.02^ef

22.64 ± 0.48^b₂

0.71 ± 0.00^e

0.71 ± 0.00^c

M6

B5 + 4 mg/L NAA + 1 mg/L BAP

0.73 ± 0.02^bc

15.85 ± 0.07^d₂

1.22 ± 0.007^d

0.71 ± 0.00^c

M7

B5 + 4 mg/L NAA + 0.1 mg/L BAP

0.70 ± 0.02^bc

14.23 ± 0.17^e₂

1.87 ± 0.015^b

1.22 ± 0.009^b

M8

B5 + 4 mg/L NAA + 0.5 mg/L BAP

0.60 ± 0.02 ^cd

13.00 ± 0.14^f₂

0.71 ± 0.00^e

0.71 ± 0.00^c

M9

B5 + 4 mg/L NAA + 0.1 mg/L BAP + 1 g/L glutamine

0.49 ± 0.02^de

16.55 ± 0.29^d₂

0.71 ± 0.00^e

0.71 ± 0.00^c

M10

B5 + 4 mg/L NAA + 1 mg/L BAP + 1 g/L glutamine

0.41 ± 0.02^ef

14.24 ± 0.33^e₂

0.71 ± 0.00^e

0.71 ± 0.00^c

M11

Double layer media—MS + 2%sucrose, 0.5% activated charcoal, gelrite- 2.6 g/L, Liquid upper layer contained half strength MS + 2% sucrose

0.31 ± 0.02 ^fg

11.29 ± 0.26^g₂

0.71 ± 0.00^e

0.71 ± 0.00^c

m12

Double layer media—Nistch medium, Maltose- 2%, Activated Charcoal – 0.5%, gelrite- 2.6 g/L, Liquid top layer consists of Nistch medium and 2% maltose

0.26 ± 0.02 ^g

10.67 ± 0.25^g₂

0.71 ± 0.00^e

0.71 ± 0.00^c

CV

3.56

2.82

1.11

0.56