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Botanical Studies
Fig. 3 | Botanical Studies

Fig. 3

From: An optimum study on the laser scanning confocal microscopy techniques for BiFC assay using plant protoplast

Fig. 3

Protoplast with weak BiFC signal that appeared concomitantly with chloroplast signal. A DAPI fluorescence. B Fluorescence of YFP and chloroplasts. C Bright filed image of the protoplast. D Autofluorescence of chloroplasts. E Merged image of A, B and D. F Merged image of A–D. Dotted circle shows signals from the nucleolus. All images are shown at the same magnification. Scale bar = 5 µm

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