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Botanical Studies
Fig. 6 | Botanical Studies

Fig. 6

From: An optimum study on the laser scanning confocal microscopy techniques for BiFC assay using plant protoplast

Fig. 6

The correlation between inner diameter of pipette tips and protoplast intactness. A The broken ratio of protoplasts decreased when protoplast samples were pipetted using tips with cut ends. B The inner diameter of different tip ends. The broken ratio of protoplasts was calculated using the equation: broken protoplasts/total protoplasts. Protoplast samples were pipetted at a speed of 2–3 s per pipetting action. The test was repeated three times

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