Fig. 3

Impact of autophagy inhibitors on lipid body redistribution. Dynamic redistribution of pollen grain lipid bodies during 1–2 h of germination in the germination medium, as revealed by BODIPY 493/503 staining and Confocal fluorescence microscopy. The effects of pre-treatment with autophagy inhibitors on mature pollen germination are depicted. Arrows indicate the locations of pollen tubes. (A): Pollen grains before germination. (A1) Distribution of lipids within pollen observed using BODIPY 493/503 staining. (A2) Merged image illustrating lipid distribution within the cell. (B): Pollen grains after 1 h of germination in germination medium (GM). (C, D, E): Pollen grains pre-treated with 3-MA, NEM, and BFA, respectively, followed by 1 h of germination observation. (F): Untreated pollen grains germinated in a culture medium for 2 h. (G, H, I): Pollen grains pre-treated with 3-MA, NEM, and BFA, respectively, followed by 2 h of germination observation. Scale bar: 20 μm