Fig. 6
From: Dynamic organelle changes and autophagic processes in lily pollen germination
Presence and subcellular localization of ATG-8 in germinating lily pollen. (A): Total proteins extracted from Arabidopsis seedling (At) or 60-minute germinating lily pollen (Lily) were subjected to immunoblotting analysis using anti-ATG8 antiserum, revealing multiple recognized bands by the anti-ATG8 antiserum (left panel). Immunoblotting analysis with affinity-purified anti-ATG8 antiserum identified a single major ATG8 protein in Arabidopsis seedlings and germination lily pollen (right panel in A). Desiccated lily pollen grains, germinated for 60 min in a culture medium, were embedded in LR White resin for subsequent immunocytochemical examination employing control (secondary antibody only, B) and affinity-purified anti-ATG8 antiserum (C-D). Only a minimal number of gold particles were recognized by the secondary antibody (B). Numerous ATG8 orthologous proteins were detected in tubular ER structures surrounding amyloplasts (arrows in C-D), oil bodies (arrow in E), and the degraded amyloplast/ starch granules (s) within the vacuolar lumen (v, in F). Legend Key: a: amyloplast, er: endoplasmic reticulum, l: lipid body, m: mitochondria, S: starch. Scale Bar: 0.5 μm